ABI Prism 7900HT Sequence Detection System

我们需要ABI Prism 7900 HT序列检测系统及其自动化附件。该仪器将存放在分子医学研究所的载体开发核心实验室。参与这项资助申请的研究人员包括10名NIH资助项目的研究人员和3名潜在的年轻研究人员。研究人员来自德克萨斯大学休斯顿分校的分子医学研究所和医学院，以及德克萨斯A&M大学的生物科学和技术研究所。所涉及的研究项目多种多样，从基础生物学、癌症研究、各种疾病中靶基因的鉴定、基因治疗、人类和动物组织中差异基因表达的研究，到涉及硬皮病和中风易感性基因分型的临床研究。我们打算使用该仪器的高通量实时定量分析生物样品中的RNA转录本和DNA靶标，并用于单核苷酸多态性基因分型。正如研究项目中所指出的那样。从每个研究者的角度来看，拟议的工具的可用性将大大改善每个人的研究范围。它将使我们能够迅速跟进我们目前资助的项目中令人兴奋的发现。这一多学科合作努力的目标是开发和共享开展尖端科学工作所需的先进技术资源，这些工作将成为未来医学研究的特征。最重要的是，现有的研究人员将能够向学生和医学研究员提供这种强大的工具，并有一个新的生物工具的环境，年轻的潜在研究人员可以在其中发展自己的科学研究。所要求的仪器是高通量实时PCR系统，可检测和定量核酸序列。具有96或384孔板容量的自动化附件便于24小时无人值守操作。集成的条形码阅读器简化了样品跟踪。7900 HT系统是一种操作非常简单的仪器。整个RT-PCR运行将在2小时内完成，然后个人可以检索他/她的结果，并使用安装在他/她自己的计算机中的软件分析数据。ABI Prism序列检测系统已被证明是一个稳定和易于操作的。这一工具的可用性对于改进我们目前资助的项目至关重要。

We are requesting an ABI Prism 7900HT Sequence Detection System with the automation accessary. This instrument will be housed in the Vector Development Core Laboratory at the Institute of Molecular Medicine. The group of investigators participating in this grant application includes ten researchers with NIH-supported grant projects and three potential young investigators. The investigators are from the Institute of Molecular Medicine and the Medical School from UT- Houston and the Institute of Biosciences and Technology from Texas A&M University. The research projects involved are diverse, ranging from basic biology, cancer research, the identification of target genes in various diseases, gene therapy, and investigating differential gene expression in tissues from human and animals to clinical studies involving the genotyping of susceptibility to Scleroderma and stroke. We intend to use this instrument for the high-throughput real-time quantitative analysis of RNA transcripts and DNA targets in biological samples and for single nucleotide polymorphism genotyping. As indicated in the research project. from each investigator, the availability of the proposed instrument will significantly improve the scope of each one's study. It will allow us to rapidly follow up the exciting discoveries from our currently funded projects. The goal of this multidisciplinary collaborative effort is to develop and share the advanced technology resources needed to carry out the cutting-edge scientific work that will characterize medical research of the future. Most importantly, the established investigators will be able to provide exposure to students and medical fellows to this powerful instrument and to have an environment with new biological tools in which young potential investigators can develop his/her own scientific research. The requested instrument is a high-throughput real-time PCR system that detects and quantifies nucleic acid sequences. An automation accessory with a 96- or 384-well plate capacity facilitates 24-hour unattended operation. The integrated bar code readers simplify sample tracking. The 7900HT system is a remarkably simple instrument to operate. The entire RT-PCR run will be completed in 2 h and the individual can then retrieve his/her results and analyze the data using the software installed in his/her own computer. The ABI Prism Sequence Detection System has been proven to be a stable and easy to operate. The availability of this instrument is essential for improving our current funded projects.