Fluorescence, Chemiluminescence and Phosphor Imager

一个分子动力学台风8600可变模型凝胶和印迹成像系统被要求支持在德克萨斯大学健康科学中心圣安东尼奥（UTHSCSA）的研究人员正在进行和未来的研究。该仪器将支持需要最先进的图像分析仪器的研究，以研究致力于了解人类疾病基本过程的各种研究项目中的分子和细胞功能。拟议的仪器将大大提高从医学和研究生院的四个不同部门的九个主要用户的研究。这一多样化用户群体的共同特征包括：1）对现场荧光、荧光和存储磷光体成像能力的迫切需求，2）致力于了解与人类疾病相关的基本生物学问题的研究项目，以及3）NIH资助的对放射性、荧光和荧光样品的快速、灵敏和灵活的数字成像有特定需求的研究项目。拟议的文书将大大加强对以下具体目标的研究：1）免疫球蛋白类别转换，2）迷走毛滴虫病发病机制，3）NADPH氧化酶和炎症，4）沙门氏菌病发病机制和抗微生物肽，5）霍乱弧菌病发病机制，6）霍乱弧菌病发病机制，（胰岛素受体信号传导，7）新生儿呼吸窘迫综合征（Mustafa），8）B细胞发育和寄生虫免疫，和9）致病真菌毒力的遗传学。台风8600得到了该机构的大力支持。该大学将提供实验室空间和资源，用于仪器维护和服务，该仪器将成为核酸技术核心设施的重要组成部分。该核心目前提供DNA测序与数据分析和引物设计，定制DNA/RNA合成，基因扩增服务和机器人应用。台风的加入将大大提高这一核心设施的能力，并为UTHSCSA和南德克萨斯州的研究计划提供巨大的好处。

A molecular Dynamics Typhoon 8600 variable model gel and blot imaging system is requested to support the ongoing and future studies of researchers at the University of Texas Health Science Center at San Antonio (UTHSCSA). This instrument will support research that requires state-of-the-art image analysis instrumentation to study molecular and cellular function in a diverse array of research projects devoted to understanding fundamental processes in human disease. The proposed instrument will greatly enhance the research of nine major users from four different departments from the Medical and Graduate Schools. Common features of this diverse group of users include: 1) an immediate need for on site fluorescent, chemiluminescent and storage phosphor imaging capabilities, 2) research projects committed to understanding fundamental biological questions relevant to human disease, and 3) NIH-funded research programs that have specific needs for rapid, sensitive and flexible digital imaging of radioactive, fluorescent and chemiluminescent samples. Research in the following specific aims will be significantly enhanced by the proposed instrument: 1) Immunoglobulin class switching, 2) Trichomonas vaginalis pathogenesis, 3) NADPH oxidases and inflammation, 4) Salmonella pathogenesis and anti-microbial peptides, 5) Vibrio Cholerae pathogenesis, 6( Insulin receptor signaling, 7) Neonatal respiratory distress syndrome (Mustafa), 8) B cell development and parasite immunity, and 9) Genetics of virulence in pathogenic fungi. The Typhoon 8600 has the strong endorsement of the institution. The University will provide laboratory space and resources for instrument maintenance and service and the instrument will be an essential component of the Nucleic Acids Technology Core facility. This core currently offers DNA sequencing with data analysis and primer design, custom DNA/RNA synthesis, gene amplification services, and robotic applications. The addition of the Typhoon will significantly enhance the capabilities of this core facility and provide great benefit to research programs at UTHSCSA and in South Texas.