Neural Substrates of Arousal and Emotion

描述（由申请人提供）：血清素，作为一种化学品 中枢神经系统（CNS）中的神经递质， 在各种人类神经和精神病理学中的作用然而，其基本 神经生物学作用仍然有些模糊。一个真正欣赏如何 5-羟色胺在疾病过程中的作用以及对它们的治疗， 了解其在正常哺乳动物生理和行为中的作用。到 为此，目前的研究旨在进一步了解基本的 中枢神经系统5-羟色胺系统的功能主要通过记录 行为动物大脑5-羟色胺神经元的电活动。录音 将在5-羟色胺神经元的吻侧/上行组（在 中缝背核）以及尾/降组（在核中 暗缝和苍白球）。拟议的研究是我们的一个产物， 一个基本假设，即血清素神经元的活动与 行为激活/强直性运动活动水平，尤其是 重复性运动活动（中枢模式发生器介导）。两大 实验内容包括：1）环境因素和行为因素 可以调节这些脑细胞的基本活动， 调节这些作用的神经化学物质; 2）大脑血清素的作用 神经元中枢疲劳后一个问题的重要性源于 事实上，疲劳被认为是一个重要组成部分，在许多 疾病过程（例如，慢性疲劳综合征，多发性硬化症， 抑郁症）。除了在行为动物中的单个单元记录外， 实验将采用：1）脑5-羟色胺的体内微透析测量 2）通过反向微透析进行局部给药; 3)c-Fos表达（结合双标记）作为 在特定条件下激活的5-羟色胺神经元;以及4） 计算机生成的尖峰序列分析。

DESCRIPTION (provided by applicant): Serotonin, acting as a chemical neurotransmitter in the central nervous system (CNS), is importantly implicated in a variety of human neuro- and psychopathologies. However, its basic neurobiological role remains somewhat obscure. A true appreciation of how serotonin functions in disease processes, and treatment of them, presupposes an understanding of its actions in normal mammalian physiology and behavior. To that end, the present research is aimed at furthering knowledge of the basic functioning of the CNS serotonin system primarily through recording the electrical activity of brain serotonin neurons in behaving animals. Recordings will be made in both the rostral/ascending group of serotonin neurons (in the dorsal raphe nucleus) as well as the caudal/descending group (in the nuclei raphe obscurus and pallidus). The proposed studies are an outgrowth of our basic hypothesis that the activity of serotonin neurons is closely linked to level of behavioral activation/tonic motor activity, and especially to repetitive motor activity (central pattern generator-mediated). Two major groups of experiments are proposed: 1) environmental and behavioral factors that may modulate the basic activity of these brain cells, and the neurochemicals that mediate these effects; 2) the role of brain serotonin neurons in central fatigue. The importance of this latter issue derives from the fact that fatigue is considered an important component in a number of disease processes (e.g., chronic fatigue syndrome, multiple sclerosis, and depression). In addition to single unit recordings in behaving animals, the experiments will employ: 1) In vivo microdialysis measures of brain serotonin and dopamine; 2) Local drug administration by means of reverse microdialysis; 3) c-Fos expression (in conjunction with double labeling) as an indicator of serotonin neurons activated under specific conditions; and 4) Computer-generated spike train analyses.