Core--Morphology and immunohistochemistry facility

了解视网膜和肾脏中哪些细胞类型受到糖尿病的影响对于有效的靶向治疗至关重要。特定蛋白质和细胞死亡标记的细胞定位，以及治疗对视网膜和肾脏结构的影响的定量分析是当前提案中项目的组成部分。具体目标1：形态学和免疫组织化学核心的目的是通过提供：a.组织收集和处理的技术支持；b.视网膜和肾脏组织病理学评估的专家协助；c.定量免疫组织化学试剂和技术支持，来帮助实施基于解剖学的实验。具体目标2：形态学和免疫组织化学核心将通过为项目提供专家援助和特殊设施来促进和刺激个别项目的研究，这些项目是必不可少的，但超出了研究人员目前的能力。它还将提供质量控制和促进研究人员之间的互动，并在这些技术领域提供教学。

Morphology and Immunohistochemistry Core Scientific Abstract An understanding of which cell types within the retina and kidney are affected by diabetes is essential for effective treatment targeting. The cellular localization of specific proteins and cell death markers, and the quantitative analysis of effects of therapies on retinal and renal structure are integral parts of the projects in the current proposal. Specific Aim 1: The purpose of the Morphology and Immunohistochemistry Core is to aid in the execution of anatomically- based experiments by providing: a. technical support for tissue collection and processing, b. expert assistance in evaluation of retinal and renal histopathology, and c. quantify reagents and technical support for immunohistochemistry. Specific Aim 2: The Morphology and Immunohistochemistry Core will facilitate and stimulate research in individual projects by providing expert assistance and special facilities essential for the projects but beyond the current capabilities of investigators. It will also provide quality control and facilitate interaction between investigators, and provide teaching in these technical areas.