CORE--FLUORESCENCE LABORATORY

描述：该核心将提供对设备的访问，以及 关于荧光定量测定的正确用法培训 在活细胞中。因为有些仪器很贵，而且 设置起来很耗时，其目标是提供一个公共设施来 支持单项研究项目。当前的两种设备 将在这个核心中使用：a)计算机控制的荧光计 用于测定细胞群体中的荧光，或者在 悬浮液或单层；b)基于显微镜的数字成像 系统。这两种类型的设备都将由调查人员在他们 都接受过核心总监的培训。来自全国各地的调查人员 项目需要以这样或那样的形式提供这些设施。

Description: This core will provide access to equipment, as well as training in proper usage, for quantitative determinations of fluorescence in living cells. Because some of this instrumentation is expensive and time-consuming to set up, the goal is to provide a common facility to support individual research projects. Two types of equipment in current use will be available in this core: a) A computer-controlled fluorometer for determination of fluorescence in populations of cells, either in suspension or in monolayers; and b) a microscope-based digital imaging system. Both types of equipment will be used by investigators after they have been trained by the Core Director. Investigators from all of the projects have need for these facilities in some form or other.