Core--Microarray analysis

描述：(申请人描述)监测基因表达模式 使用Edna片段的微阵列网格是一种强大的新工具 分析肿瘤。设计和准备微阵列的内部能力将 让我们有机会量身定制低成本基因芯片的设计 黑色素瘤计划项目的需求。昂贵的商业芯片(这 目前每个电网包含15,000个EDN，预计很快将包括 代表大多数或全部表达的人类基因的DNA)可以购买并 很少用于非常精选的实验和有趣的cdna克隆 从这些“全球”实验的结果中辨认出来的 包含在更小、更便宜、更容易获得的内核上 微阵列。这些新颖的微阵列设计策略将避免巨大的 日常使用商业基因芯片可能招致的费用，以及 将通过简化数量基因来促进数据解释 将表达式读出到已知的有限(但仍然非常大)的EDNA集合 或期望在黑色素瘤中以有趣的方式波动。 微阵列将广泛用于4个项目中的3个。核心E将 与核心D的蛋白质组分析组件密切互动，以便 为一些项目的定制微阵列设计提供便利。

DESCRIPTION: (Applicant's Description) Monitoring gene expression patterns using microarrayed grids of eDNA fragments is a powerful new tool for analyzing tumors. An in-house capacity to design and prepare microarrays will give us the opportunity to tailor the design of low-cost gene chips to the needs of the melanoma program project. Expensive commercial chips (which presently contain >15,000 eDNAs per grid and are expected to soon include cDNAs representing most or all expressed human genes) can be purchased and used sparingly for very selected experiments, and interesting cDNA clones identified from the results of these "global" experiments can he selected for inclusion on the smaller, cheaper, and more readily available core-generated microarrays. These novel microarray design strategies will avoid the huge expense likely to be incurred by the routine use of commercial gene chips, and will facilitate data interpretation by simplifying the quantitative gene expression readout to a limited (but still very large) set of eDNAs known to or expected to fluctuate in interesting ways in melanoma. Microarrays will be used extensively for 3 of the 4 projects. Core E will interact closely with the proteome analysis component of Core D in order to facilitate the design of custom microarrays for some of the projects.