Core--Tissue culture laboratory

集中式组织培养设施比单个研究人员进行自己的细胞培养更经济。拟议的核心将是适应这一计划项目的组织培养核心的扩展，该核心自1980年以来一直在内分泌科运行。建立和维护共同的组织培养核心的原因包括：1.细胞系、培养技术和解决方案的质量控制2.大量制备细胞和培养基能带来经济优势3.最佳利用时间、设备和用品4.经验丰富的技术人员5.准备之间的一致性集中式组织培养设施是维持细胞系的最有效手段。这些细胞系被编目并作为备用库存储存在装有警报系统的液氮储罐中，以防止重要细胞系的损失。所有细胞系每年都要进行两次支原体检测。批量购买胎牛血清和培养液肯定更划算。在购买前对3到4个来源的血清进行预筛选，以实现最佳细胞生长，这在核心设施中是最有效的。媒体和PBS可以一次准备10升、20升或30升，然后在使用前进行测试。批量订购塑料制品也更具成本效益。在特定的预定时间，定期检查和维护保温箱和罩子。

A centralized tissue culture facility is more economical than individual investigators perform their own cell culture. The proposed core would be an expansion to accommodate this program project of a tissue culture core that has been in operation since 1980 in the Division of Endocrinology. Reasons for establishing and maintaining a common tissue culture core include the following: 1. Quality control of cell lines, culture techniques, and solutions 2. Bulk preparation of cells and media gives an economical advantage 3. Optimal use of time, equipment and supplies 4. Experienced technicians 5. Consistency between preparations A centralized tissue culture facility is the most efficient means for maintaining cell lines. The cell lines are catalogued and frozen as back-up stocks in liquid nitrogen tanks equipped with alarm systems to prevent loss of important cell lines. All cell lines are screened for mycoplasma detection twice per year. Purchase of fetal calf serum and media in bulk is certainly more cost-effective. Prescreening of 3 to 4 sources of serum for optimal cell growth before purchase is most efficiently accomplished in the core facility. Media and PBS can be prepared at 10, 20, or 30 liters at a time and then tested before use. Bulk ordering of plasticware is also more cost effective. The incubators and hoods are checked and maintained on a periodic basis at specific scheduled times.