Investigating the mode of actionand target proteins of novel herbicide leadsusing the Marchantia polymorphamodel system

在现代农业中，使用除草剂来控制作物杂草种群是一种常见的做法。这一过程旨在通过减少杂草的存在造成的损失来提高产量，杂草与农作物争夺光、水和养分等资源。然而，长期广泛使用有限范围的除草剂可能会降低除草剂的使用效果。这是因为许多杂草物种已经对农民使用的有限数量的除草剂产生了抗性。过去30年里，没有新的除草剂行动模式推向市场，这加剧了农民除草剂选择数量的减少。解决除草剂抗药性问题的一种方法是开发新的除草剂，这种除草剂具有新的作用模式，但没有抗药性。除草剂的发现率比以前低得多，然而，随着新发现方法的发展，新的具有除草活性的化合物开始被发现。MoA Technology公司使用高通量体内方法鉴定了几种具有除草活性的化合物。该项目旨在确定目前正在开发的一些新型除草剂如何杀死植物。这将在模拟陆地植物系统Marchantia Polyma中使用各种方法完成。详细了解这些化合物如何影响植物生长，特别是Marchantia的孢子发育，将增加MOA技术将一种有效的商业除草剂推向市场的机会。此外，详细分析这些先导化合物的选择如何影响其靶标，以及这个靶标在野生型品系中的功能，将增加我们管理这些化合物的抗性发展的能力，如果它们有一天在田间使用的话。增加新除草剂的寿命将确保农民可以获得的新发现的除草剂品种得以保持，从而使高效的粮食生产能够满足未来日益增长的需求。

The application of herbicides to control populations of weeds in crops is common practice in modern agriculture. This process aims to increase yields by reducing losses caused by the presence of weeds, which compete with crops for resources such as light, water and nutrients. However, the extensive use of a limited range of herbicides over long periods of time threatens to reduce the efficacy of herbicide application. This is because many weed species have evolved resistance to the limited number of herbicides used by farmers. The reduction in the number of herbicide options for farmers has been exacerbated by no new herbicide mode of action being brought to market in the last 30 years. One way in which the herbicide resistance problem canbe combatted is by developing new herbicides with novel modes of action to which there is no resistance. Herbicide discovery rates are far lower than they once were, however, with the development of new methods of discovery, new compounds with herbicidal activity are beginning to be uncovered.The company,MoA Technology,has identified several compounds with herbicidal activity using a high-throughput in vivo method. This project aims to determine how some of these novel herbicides which are currently in development kill plants. This will be done using a variety of methods in the model land plant system Marchantia polymorpha. A detailed understanding of how these compounds affect plant growth, and in particular spore development in Marchantia, will increase the chances of MoA Technology bringing an effective commercial herbicide to market. Furthermore, a detailed analysis of how a selection of these lead compounds affect their target, and the function of this target in wild-type lines, will increase our ability to manage the development of resistance to these compounds if they are one day used in the field. Increasing the longevity of new herbicides will ensure the new found variety of herbicides available to farmers will be maintained, allowing efficient food production to feed a growing demand in the future.