STRUCTURE OF DNA POLYMERASE OF HYPERTHERMOPHILE PYROCOCCUS FURIOSUS (PFU)

DNA聚合酶是负责复制 细胞中DNA分子的基因组和修复。中温型和中温型 嗜热聚合酶蛋白已经被解决，但还没有完全 从一个嗜热者身上提取的聚合酶已经完成。结构性的 来自已解决的晶体的数据将提供更多的数据以供改进 他们对聚合酶结构的理解 如DNA结合两种核酸外切酶活性和 聚合酶活性。这种结构可能有助于扩展我们的基本 对分子内相互作用的理解 这种超嗜热性蛋白质的热稳定性。

DNA polymerase is the enzyme responsible for the replication of the genome and repair of DNA molecule in cells. Both mesophilic and thermophilic polymerase proteins have been solved but no whole polymerase from a hyperthermophile has been completed. The structural data from a solved crystal would give more data with which to improve their understanding of the polymerase structure with respect to its activities such as DNA binding the two exonuclease activities and polymerase activity. The structure may help in expanding our basic understanding of intramolecular interaction which contribute to the thermostability of this hyperthermophilic protein.