CENTER FOR GENOME RESEARCH

在NHGRI的测序试点项目下，怀特黑德/麻省理工学院测序中心 基因组研究开发了一种可扩展的高效流程， 基因组测序 这项建议的具体目的是将我们的试点项目转变为一个 生产中心： (i)通过扩大我们的车道，将车道容量提高到每年2000万条车道， 用于样品制备和购买毛细管的自动化系统 定序器;以及 (ii)达到至少750的成品序列的生产率 Mb/年，成本为10美分/基，包括间接费用。 我们还致力于与NHGRI和其他中心密切合作 以适应快速变化的情况，以确保整体快速 人类基因组计划的成功

Under NHGRI's sequencing pilot project, the Whitehead/MIT Center for Genome Research has developed a scalable and efficient process for genomic sequencing. The specific aim of this proposal is to convert our pilot project to a production center by: (i) increasing lane capacity to 20 M lanes per year, by expanding our automated systems for sample prep and purchasing a fleet of capillary sequencers; and (ii) achieving a production rate of finished sequence of at least 750 Mb per year at a cost of 10 cents/base including overhead. We are also committed to working closely with NHGRI and other centers to adapt to rapidly changing circumstances, to ensure the overall rapid success of the Human Genome Project.