CENTER FOR REPRODUCTIVE SCIENCES

堪萨斯大学医学中心提议建立一个U 54生殖研究专业合作中心，解决与男性、女性和早孕建立相关的不孕症的尖端分子和细胞问题。三个核心和四个研究项目是由杰出的和著名的生殖生物学家谁致力于他们的职业生涯在这一领域提出的。项目I，SF-1在性腺中表达的调节，将使用啮齿动物模型系统来鉴定调节睾丸中SF-1表达的反式作用因子，表征调节SF-1表达的转录因子USF 1和2，并鉴定SF-1正确时空表达所需的Ftz-Z1基因的最小区域。SF-1在性腺发育和类固醇生成中的关键性质强调了鉴定导致SF-1诱导的事件和对SF-1活性有反应的事件的重要性。项目II，Src酪氨酸激酶调节卵巢功能，将在小鼠和人类中研究其在卵巢卵泡发育机制中的作用，Src酪氨酸激酶通过该机制调节卵巢细胞对促性腺激素的反应。项目III，着床过程中子宫血管变化和血管生成方面将在小鼠中测试以下假设：血管内皮细胞生长因子及其受体和神经纤毛蛋白-1是着床和蜕膜化所需的血管通透性和血管生成的重要介质。此外，据推测，环氧合酶-2缺失小鼠的着床失败是这些基因在关键的围着床期子宫异常表达的结果。项目IV，TRAIL和人类植入部位，将研究滋养层细胞TRAIL及其受体在调节植入部位的免疫相互作用以及指导早期人类胎盘的发育和功能中的作用。细胞培养和组织分析核心和成像核心是完善的，具有成本效益的核心，将作为开放获取运作，因为有许多NICHD批准的核心赠款，补充了U 54项目。行政核心将促进科学交流，并协助管理U 54核心和项目。总的来说，堪萨斯U 54中心，这一直是在这个机构的一个主要焦点超过三十年，将作为一个有效的，具有成本效益的工具，促进当代研究生育和不育。

The University of Kansas Medical Center proposes to establish a U54 Specialized Cooperative Center in Reproductive Research that addresses cutting edge molecular and cellular issues of infertility related to male, female, and establishment of early pregnancy. Three cores and four research projects are proposed by outstanding and well-known reproductive biologists who have dedicated their careers to this field. Project I, Regulation of SF-1 Expression in the Gonads, will use a rodent model system to identify transacting factors regulating SF-1 expression in the testis, characterize the transcription factors, USF1 and 2, regulating SF-1 expression, and identify the minimal region of the Ftz-Z1 gene required for correct spatial and temporal expression of SF-1. The pivotal nature of SF-1 in gonadal development and steroidogenesis underscores the importance of identifying events leading to SF-1 induction and those responsive to SF-1's activity. Project II, Src Tyrosine Kinase Regulates Ovarian Function, will examine in mice and humans and its role in ovarian follicular development mechanisms by which Src tyrosine kinase regulates responses of ovarian cells to gonadotropins. Project III, Aspects of Uterine Vascular Changes and Angiogenesis during Implantation will test in mice the hypothesis that vascular endothelial cell growth factor, its receptors and neuropilin-1 are important mediators of vascular permeability and angiogenesis required for implantation and decidualization. In addition, it is hypothesized that failure of implantation in cyclooxygenase-2 null mice is the result of aberrant uterine expression of these genes during the critical peri-implantation period. Project IV, TRAIL and the Human Implantation Site, will investigate the role of trophoblastic TRAIL and its receptors in modulating immunological interactions at the implantation site as well as directing development and function in early human placentas. The Cell Culture and the Tissue Analysis Core and Imaging Core are well established, cost effective cores that will operate as open access since there are numerous NICHD grants approved for core access that complement the U54 projects. The Administrative Core will promote scientific exchange and assist in management of U54 cores and projects. Overall, the Kansas U54 Center, which has been a major focus in this institution for more than three decades, will serve as an efficient, cost-effective vehicle for promoting contemporary research on fertility and infertility.