CORE--MEDIA PREPARATION

描述：(申请人描述)媒体准备共享设施 UAB综合癌症中心(UAB-CCC)的MPSF是 (A)真核细胞和细菌细胞培养基及 常规电池中使用的补充剂、(B)缓冲液和其他大容量试剂 培养和分子生物学应用；(C)质量控制保证和 (D)关于细胞的一般咨询和教育 培养和分子生物学试剂。总体目标是改进 UAB-CCC研究活动的经济性、效率和质量。 MPSF通过提供共同的文化媒体、补充剂和 以低于可用容量使用计费的价格向UAB-CCC教职员工提供缓冲 商业价格。这通过以下方式实现：(1)现场生产 未能产生超额强积金收入的退还费率，以及(2)交易量 与商业供应商的采购谈判、成交价和发货 一般研究实验室无法获得的费用减免。 MPSF通过方便的现场产品/服务可用性提供效率， 与商业供应商相比，文书工作更少，交货更快。 许多用户在首次使用MPSF产品后不到2小时内即可使用 请求。 MPSF通过(A)定期进行内毒素和 现场产品无菌，以及内毒素和支原体 调查员提交的样本和现场高压灭菌器性能，以及(B) 提供个性化服务，包括专业咨询 免费解决具体实验问题，外加设计和准备 低成本定制试剂。

Description:(Applicant's Description) The Media Preparation Shared Facility (MPSF) of the UAB Comprehensive Cancer Center (UAB-CCC) serves as a centralized resource for (a) eukaryotic and bacterial cell culture media and supplements, (b) buffers and other high-volume reagents used in routine cell culture and molecular biology applications, (c) quality control assurance and testing, and (d) general consultation and education with regard to cell culture and molecular biology reagents. The overall goals are to improve economy, efficiency and quality of UAB-CCC research activities. MPSF provides economy by supplying common culture media, supplements and buffers to UAB-CCC faculty at charge-back prices lower than available commercial prices. This is accomplished via (1) on-site production with charge-back rates that fail to generate excess MPSF income, and (2) volume purchase negotiations with commercial suppliers, yielding price and shipping charge reductions not available to the average research laboratory. MPSF offers efficiency by convenient on-site product/service availability, with less paperwork and faster delivery as compared to commercial suppliers. Many users take possession of MPSF products in less than 2 hours after initial request. MPSF promotes quality by (a) regularly performing assays for endotoxin and sterility in on-site products, as well as for endotoxin and mycoplasma in investigator-submitted samples, and on-site autoclave performance, and (b) offering personalized services including professional consultation for specific experimental problems at no cost, plus design and preparation of custom reagents at low cost.