CORE--HYBRIDOMA/CELL CULTURE/IMMUNE MONITORING

MCC的杂交/细胞培养/免疫监测共享资源自1983年以来一直活跃。最初，这个核心实验室的建立是为了创造杂交瘤和为VCU的癌症研究科学家生产单克隆抗体。该技术对于几种不同的研究方向是关键的，包括癌症的鉴定和纯化-对于几种不同的研究方向是关键的，包括癌症相关蛋白的鉴定和纯化以及这些蛋白质和其他分子在细胞和组织中的定位。在过去的十年中，随着单克隆抗体和杂交瘤细胞系越来越多地从商业来源获得，创建新杂交瘤的需求有所减少。同时，这一共享资源已将其作用扩展到包括单克隆抗体的生产、杂交瘤以外的细胞系的大规模培养、用于过继免疫治疗的大量淋巴细胞的生长、所有类型细胞系的支原体检测、用于与临床试验相关的相关研究的血液样品的收获和制备，以及采集和制备血液和淋巴细胞样品，用于与临床免疫治疗试验相关的终点的免疫学监测。与最初的杂交瘤生产服务一样，所有这些功能都是为癌症研究人员服务的，对他们来说，在每个单独的实验室建立相同的能力是繁琐和低效的。虽然优先考虑受资助的癌症中心研究人员，但所有VCU科学家都能够获得该设施的服务。这一共享资源的资金将从CCSG和旨在收回供应成本的用户费用系统中获得，同时仍为VCU和癌症中心的研究科学家提供非常具有成本效益的服务。

The Hybridoma/Cell Culture/Immune Monitoring Shared Resource of the MCC has been active since 1983. Initially, this core laboratory was established for the sole purpose of creating hybridomas and producing monoclonal antibodies for cancer research scientists at VCU. This technology has been critical for several different lines of investigation, including identification and purification of cancer-critical for several different lines of investigation, including identification of purification of cancer-related proteins and localization of these proteins and other molecules in cells and tissues. Over the past decade, as monoclonal antibodies and hybridoma cell lines have become more available from commercial sources, the need to create new hybridoma has decreased somewhat. Simultaneously, this shared resource has expanded its role to include production of monoclonal antibodies, large scale culture of cell lines other than hybridomas, growth of large numbers of lymphocytes for adoptive immunotherapy, mycoplasma testing of cell lines of all types, harvest and preparation of blood samples for correlative studies associated with clinical trials, and harvest and preparation of blood and lymphocyte samples for immunologic monitoring of endpoints associated with clinical immunotherapy trials. As with the original hybridoma production services, all of these functions serve cancer research investigators for whom it would be cumbersome and inefficient to establish the same capabilities in each of their separate laboratories. While preference is given to funded Cancer Center researchers, all VCU scientists are able to access the facility's services. Funding of this shared resource will be obtained from the CCSG and from a system of user fees designed to recover cost of supplies, while still providing a very cost- efficient service to research scientists at VCU and the Cancer Center.