Federally Registered Human Embryonic Stem Cell Center

描述(由申请人提供)：随着人类胚胎干细胞(HES)的去化，以及随后对那些在2001年8月9日之前衍生的品系获得联邦资助的资格的限制，合格品系的有效分布是首要的。这项提议寻求通过三个目标来实现这一目标。目的1：首先，来自加州大学旧金山分校(UCSF)、HSF-6和HSF-1的HES细胞将进行大规模扩增，进行无菌测试、HES细胞特性和分化潜能测试；以便分布到世界各地的学术机构。被选为有用特征的HES品系的亚系，如绿色荧光蛋白的表达和HES细胞表面标记的高水平表达，也将用于分布。一个网站将向用户提供关于细胞系的信息和订单。目标2：其次，将启动一项针对合格技术人员的培训计划，为受援实验室提供在其自己的实验室成功建立HES培养所需的专业知识。最后，加州大学旧金山分校现有的HES细胞系将逐步转移到包含良好实验室实践的培养中，包括对细胞历史和培养条件进行广泛的测试和记录。这些目标的实现将为使用者提供必要的宝贵工具，以进行人类发育和人类移植组织生成的创新研究。

DESCRIPTION (PROVIDED BY APPLICANT): With the dervation of human embryonic stem (hES) cells, and the subsequent limitation of eligibility for federal funding to those lines derived prior to August 9, 2001, the efficient distribution of the eligible lines is of primary importance. This proposal seeks to achieve this goal through three aims. Aim 1: First, The hES cells derived at University of California, San Francisco (UCSF), HSF-6 and HSF-1 will be expanded in large lots, tested for sterility, hES cell characteristics, and differentiation potential; for distribution to academic facilities throughout the world. Sublines of hES lines selected for useful characteristics, such as expression of green fluorescent protein and high levels of expression of hES cell-surface markers will also be derived for distribution. A website will provide users with information about the cell lines, and order forms. Aim 2: Secondly, a training program will be initiated for qualified technical staff to provide recipient labs with the expertise necessary to successfully establish hES cultures in their own lab. Finally, the hES cell lines available at UCSF will be gradually transferred to cultures incorporating good laboratory practices, including extensive testing and documentation of the history of the cells and culture conditions the lines have encountered. Achievement of these aims will provide users with the valuable tools necessary to perform innovative studies of human development and generation of tissues for human transplantation.