Lectin based open tubular micro-reactors for probing protein-protein binding

该项目旨在开发非侵入性技术来测量和定量凝集素-碳水化合物结合事件，首先是糖肽和糖蛋白。其目标不仅是在开发的系统中提供准确的蛋白质-蛋白质缔合和解离常数数据，而且还在封闭的微流体环境中做到这一点，另外还有一个优势，即还提供“捕获和释放”提取能力，并且易于与色层和质谱分析系统耦合。

This project seeks to develop non-invasive technology for the measurement and quantitation of lectin-carbohydrate binding events, in the first instance glycopeptides and glycoproteins. The goal is not only to provide accurate protein-protein association and dissociation constant data within the developed system, but to do so within an enclosed micro-fluidic environment, with the added advantages of also providing ‘trap and release’ extraction capabilities, and being easily coupled to both chromatographic and mass spectrometry systems.