CORE--TISSUE CULTURE AND PHENOTYPE

组织和细胞的获取、制备和培养是至关重要的，但也是耗时和昂贵的面向皮肤的研究方面。通过使用组织培养和表型核心这一中心资源，该中心也成为SDRC研究人员之间互动和合作的重点场所，已经取得了大量和更高的效果。核心的目标是：(1)通过作为组织、细胞和其他试剂的来源，继续促进与皮肤相关的研究；(2)在保持质量控制的同时，通过系统和同步的组织/细胞采购和培养过程提高皮肤研究的成本效益；(3)协助中心研究员的皮肤研究，为组织表型提供技术和智力支持；(4)促进皮肤相关问题的跨学科讨论和互动，特别强调转化研究。为达致上述目标，中心将继续提供下列服务：它将：(a)作为皮肤组织的可靠来源（人类和老鼠皮肤库）；(b)纯化和培养来自人和小鼠皮肤的不同细胞群，包括皮肤病患者的活检标本（细胞库）；(c)以具有成本效益的方式提供综合的单克隆抗体、生长因子和DNA（抗体、生长因子和DNA库）；(d)大量购买组织培养试剂并建立分享机制；(e)定期监测组织和细胞的质量；(f)提供细胞和组织图象分析方面的现代仪器、规程和技术援助；(f)提供从皮肤中分离和培养细胞的技术培训；(h)组织正式和非正式讨论平台。这些服务不仅将促进成本和时间效率，高质量，以人为本的研究，还将促进前沿知识和技术在皮肤疾病研究中的应用。

Acquisition, preparation, and culturing of tissue and cells are critical, but also time-consuming and expensive aspects of skin-oriented research. Numerous and heightened effectiveness have been achieved by use of a central resource, the Tissue Culture and Phenotype Core, which has also served as focal venue for interactions and collaboration among SDRC Investigators. The Core's aims are to: (1) Continue to promote skin- related research by serving as a source of tissue, cells, and other reagents; (2) improve cost-effectiveness of skin research through systematic and synchronized tissue/cell procurement and culturing processes, while maintaining quality control; (3) Assist skin-research of Center Investigators by providing technical and intellectual assistance in tissue phenotyping; and (4) Promote inter-disciplinary discussion and interaction on skin-related problems, with special emphasis on translational research. To achieve these objectives, the Core will continue to provide the following services; it will: (a) Serve as a reliable source of cutaneous tissues (Human and Mouse Skin Banks); (b) Purify and culture different cell populations from human and mouse skin, including biopsy specimens from patients with skin diseases (Cell Bank); (c) Provide in a cost-effective manner, comprehensive, comprehensive panels of mAbs, growth factors, and DNA (Antibody, Growth Factor, and DNA Banks); (d) Purchase tissue culture reagents in bulk quantities and develop sharing mechanisms; (e) Monitor, on a regular basis, the quality of tissue and cells; (f) Provide modern instruments, protocols, and technical assistance in cell and tissue image analyses; (f) Provide training in techniques for isolating and culturing cells from skin; and (h) Organize formal and informal discussion platforms. Not only will these services promote cost- and time-efficient, high quality, sin-oriented research, they will also facilitate application of cutting-edge knowledge and technology to the investigation of skin disorders.