CORE--MORPHOLOGY

形态核心的主要目标是通过促进获取针对皮肤样本微观分析的技术，提高新的P&F调查人员和现有皮肤调查人员的多功能性和生产力。这将通过维护经验证的试剂(抗血清)库、执行免疫染色方案、筛选新的抗血清、指导/优化原位杂交方案、筛选转基因小鼠的组织学皮肤异常以及提供皮肤病理学援助来实现。这个核心将提供一个开拓性的/培育环境，在经验丰富的团队的指导下，尖端的微观技术最初得到尝试和实现。该核心将实施引入具有更高敏感性的新技术，并向更大的SDRC用户群体产生特定途径的技术集群(整合素面板、凋亡标记、信号转导探针和抗血清)，并加快从基于同位素的方案向非同位素方法的转换。该核心将及时和低成本地为显微镜标本提供最先进的包埋、切片、染色、定量形态测量、摄影布局和打印。形态核心的另一个主要功能将是获取具有准确人口统计学和诊断数据的临床衍生人类样本。对于进行皮肤相关项目的研究人员来说，持续获得大量集中的皮肤病理学和伤口愈合样本库是一个关键的组成部分和宝贵的资源。主任和所有研究助理将为P&F调查人员、国家发改委资助的调查人员及其博士后研究员、研究生和实验室人员提供有助于皮肤分析的显微技术方面的教学协助和建议。分子遗传学和表型核心之间的合作互动将在每天的基础上发生，冰冻组织被送到分子遗传学核心，或者动物样本和/或培养细胞从表型核心产生。

The primary objective of the Morphology Core is to enhance the versatility and productivity of both new P&F investigators and established skin investigators by facilitating access to techniques directed at the microscopic analysis of skin samples. This will be accomplished by maintaining banks of proven reagents (antisera), executing immunostaining protocols, screening new antisera, instructing/optimizing in situ hybridization protocols screening transgenic mice for histologic skin abnormalities, and providing dermatopathologic assistance. This core will provide a pioneering/nurturing environment where cutting edge microscopic techniques are initially tried and achieved under the guidance of an experienced team. The core will implement the introduction of new techniques with greater sensitivities and generate clusters of pathway- specific techniques (integrin panels, apoptosis markers, signals transduction probes and antisera) to the greater SDRC user group, and hasten the conversion from isotopic based protocols to non-isotopic methods. The Core will render state-of-the-art embedding, sectioning, staining, quantitative morphometry, photographic layout and printing for microscopic specimens on a timely and low-cost basis. Another prime- function of the Morphology Core will be to procure clinical-derived human samples with accurate demographic and diagnostic data. The continue availability of large, centralized bank of dermatopathologic and wound healing samples is a critical component and invaluable resource for investigators who conduct skin-related projects. The Director and all research assistants will supply teaching assistance and advice in microscopic techniques useful for skin analysis for P&F investigators, Funded SDRC investigators and their post-doctoral fellows, graduate students, and laboratory personnel. Cooperative interactions among the Molecular Genetics and Phenotype Cores will occur on a daily basis snap frozen tissues are delivered to the Molecular Genetics Core or animal samples and/or cultured cells are generated from the Phenotype Core.