Development And Diagnostic Use Of Rapid Immunoassays

几种内分泌肿瘤标志物被常规用于各种癌症的诊断和治疗。然而，目前的分析通常需要几个小时的时间才能完成，这排除了它们在患者术中管理中的使用。大多数内分泌肿瘤标志物在循环中的半衰期不到5分钟，因此，如果有快速检测方法，在手术或定位过程中监测激素浓度是可行的。这种化验的主要适应症是评估术后残留肿瘤的程度，并通过选择性静脉或动脉采样来定位肿瘤。在过去的一年里，我们通过使用针对完整蛋白的特异性抗体来改进甲状旁腺素的检测方法。我们还修改了现有的胃泌素商业测定方法，以开发一种快速免疫测定方法。在接下来的一年里，我们计划进一步发展胃泌素测定，并评估其在患者标本上的潜在临床应用。

Several endocrine tumor markers are routinely used in the diagnosis and management of various cancers. The current assays, however, typically take several hours to perform, which precludes their use in the intraoperative management of patients. Most endocrine tumor markers have a half-life in the circulation of less than 5 minutes, thus making it feasible, if a rapid assay were available, for monitoring the concentration of the hormones during surgery or localization procedures. The primary indication for such assays would be to assess the extent of residual tumor after surgery and to localize tumors by selective venous or arterial sampling. In the past year, we have improved the assay for PTH by using antibodies specific for the intact protein. We also have modified an existing commercial assay for gastrin for developing a rapid immunoassay. In the upcoming year, we plan to further develop the gastrin assay and to assess its potential clinical utility on patient specimens.