Model for Electrode Testing and Reduced Animal Use

描述（由申请方提供）：体外模型的开发， 提供有用的、具有成本效益的工具，用于评估长期电化学 提出了慢性植入电极的功能。体外模型 将为开发实验性的动物模型提供一种替代方法， 方案和评估慢性电极稳定性。他们将允许长期 电极的电化学功能和稳定性的测试 反映体内电化学环境的条件， 在动物中进行这种试验的费用和可变性。程序 专注于神经记录电极，功能性电刺激 (FES)，和中枢神经系统（CNS）中的化学传感，尽管 应用于周边电极和无源生物稳定性 植入物是预期的。一种使用鼠标的无生命模型和活体模型 提出了祖细胞。这两种模型都是为了模仿 成分和扩散环境的中枢神经系统，因为它影响 电极上的电化学。无生命模型是在第一阶段开发的。 第二阶段计划将扩大模型，包括活细胞。的 无生命和活体模型的有效性将通过比较 体外模型中的电极功能与在相同条件下观察到的电极功能相同。 慢性猫脑皮层的测试方案

DESCRIPTION (provided by applicant): The development of in vitro models that provide useful, cost-effective tools for assessing long-term electrochemical function of chronically implanted electrodes is proposed. The in vitro models will provide an alternative to animal models for developing experimental protocols and assessing chronic electrode stability. They will allow long-term testing of electrochemical function and stability of electrodes under conditions that reflect the in vivo electrochemical environment without the expense and variability of conducting such testing in animals. The program focuses on electrodes for neural recording, functional electrical stimulation (FES), and chemical sensing in the central nervous system (CNS), although applications to electrodes in the periphery and to biostability of passive implants are anticipated. An inanimate model and a living model employing mouse progenitor cells are proposed. Both models are designed to mimic the compositional and diffusion environment of the CNS as it affects electrochemistry at an electrode. The inanimate model was developed in Phase I. The Phase II program will expand the model to include living cells. The validity of the inanimate and living models will be evaluated by comparison of electrode function in the in vitro models with that observed under identical test protocols in the chronic cat cortex.