Ethnopharmacological Studies in Oral Medicine

描述（由申请人提供）：阿弗他溃疡和口腔疼痛是 被认为是人类最常见的口腔溃疡病理。 口腔皮肤病、口腔癌和口腔干燥症是显著的 相关的口腔病理，这些病症也产生显著的疼痛， 口腔内的溃疡性疾病。很少有科学研究 以受控的格式调查、确定和评估 各种植物药，这是由人类在整个地球上使用，为 口腔的各种疼痛和病理状况。最低限度的科学 可获得鉴定药物活性化合物的数据， 植物药用于治疗口腔疼痛的组织病理学作用， 溃疡和癌症病理学。 本拟议项目旨在观察、调查和确定 民族医药学口腔医学实践和口腔植物医学 这是哥斯达黎加土著人民和居民使用的制剂。 将通过文献和实地调查收集初步数据， 哥斯达黎加和美国公民的民族医药做法， 口腔的癌症、溃疡和疼痛状况。调查数据将 为目标植物的收集和编目提供指导 在哥斯达黎加和美国的口腔医学实践中使用的物种 States.将收集已鉴定的植物材料，进行分类鉴定， 提取的，在口腔相关的情感，如疼痛， 炎症、癌性生长和流涎。将建立一个协议 伊利诺伊大学芝加哥分校和拉丁美洲大学 哥斯达黎加，为这项拟议的研究获得哥斯达黎加的遗传资源。 将重新收集活性样品用于分馏和分离研究。 有前途的组成化合物将在体内和体外进行 针对表皮角质形成细胞细胞系的测试，在小鼠甩尾和 细胞毒性评估，以确定疗效、安全性和作为 镇痛、麻醉、抗炎、抗癌、抗口干 （催涎剂）药物。该提案将构成一项旨在实现以下目标的计划的基础： 植物源口服药物的发现和开发， 非微生物来源的口腔疾病和病症。

DESCRIPTION (Provided by applicant): Aphthous ulcers and oral pain are considered the most frequent oral ulcerative pathologies of the human species. Oral dermatological conditions, oral cancer and xerostomia are significant related oral pathologies and these conditions also produce significant pain and ulcerative conditions within the oral cavity. Few scientific studies exist which survey, identify and assess, in controlled formats, the efficacy of the various phytomedicines, which are used by humans throughout the planet, for the myriad pain and pathological conditions of the oral cavity. Minimal scientific data are available which identifies the medicinally active compounds and histopathological effects of phytomedicines used as treatments for oral pain, ulcer and cancer pathologies. This proposed project intends to observe, survey and identify, the ethno-pharmaceutical oral medicine practices and oral phytomedicinal preparations used by the indigenous peoples and populations of Costa Rica. Initial data will be gathered via a literature- and field-based survey of ethno-pharmaceutial practices of Costa Rican and United States citizens for cancer, ulcerative and pain conditions of the oral cavity. Survey data will provide guidance for the collection and cataloguing of the targeted plant species used during the practice of oral medicine in Costa Rica and the United States. Identified plant materials will be collected, taxonomically identified, extracted, the extracts tested in oral related affections, such as pain, inflammation, cancerous growth, and salivation. An Agreement will be set up between the University of Illinois at Chicago and the Universidad Latina of Costa Rica, to access Costa Rican genetic resources for this proposed study. Active samples will be recollected for fractionation and isolation studies. Promising constituent compounds will be subjected to in vivo and in vitro testing against epidermal keratinocytes cell lines, in mouse tail-flick and cytotoxic assessments to determine efficacy, safety, and potential as analgesic, anesthetic, anti-inflammatory, anti-cancer and anti-xerostomia (sialogogue) drugs. The proposal will form the basis of a program aimed at the discovery and development of botanically derived oral medications for non-microbial originating oral diseases and conditions.