MOLECULAR MECHANISM OF MYOSIN IX MOTILITY

我们的建议是表达和研究非常规肌球蛋白IX的功能。申请人将表达和表征非常规肌球蛋白的运动和钙调蛋白结合域。首先，将进行完整的动力学研究，然后进行低分辨率结构研究，包括低温电镜。，并试图生产用于x射线衍射的晶体。同时，将尝试进行单分子研究，以验证肌球蛋白IX是单头进程马达的假设。结构研究和单分子研究将涉及与其他实验室的合作，包括宾夕法尼亚肌肉研究所的实验室。该项目将为学员提供研究分子马达所需的各种方法，并为肌球蛋白家族的一个独特成员提出一个有趣的假设。

The proposal is to express and study the function of the unconventional myosin IX. The applicant will express and characterize the motor and calmodulin binding domains of unconventional myosin. First, a complete kinetic study will be undertaken, followed by low resolution structural studies involving cryo-e.m.,and attempts to produce crystals for X-ray diffraction. In parallel, single molecule studies will be attempted to test the hypothesis that myosin IX is a single-headed processive motor. The structural studies and single molecule studies will involve collaborations with others labs, including labs within the Pennsylvania Muscle Institute. The project will provide the trainee to a variety of approaches necessary for the study of molecular motors, as well as address an interesting hypothesis for a unique member of the myosin family.