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Midwest Consortium for High Resolution Cryoelectron Microscopy

中西部高分辨率冷冻电子显微镜联盟

基本信息

批准号：
10205086
负责人：
ESTHER BULLITT
金额：
$ 53.85万
依托单位：
PURDUE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-06-01 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10205086
关键词：
3-Dimensional Adhesions Alabama Antiviral Agents Back Bacterial Proteins Biological Process Boston Cell physiology Chemoreceptors Collaborations Collection Colorado Complex Cryoelectron Microscopy Data Data Collection Detection Development Disease Early Diagnosis Electron Beam Electron Microscope Electrons Engineering Ensure Equipment Faculty Fostering Freezing Funding Home Human Huntington Disease Image Institution Interruption Kansas Laboratories Light Macromolecular Complexes Manuals Medicine Methods Michigan Microscope Midwestern United States Missouri Motion Movement Performance Phase Productivity Proteins Recovery Research Research Personnel Resolution Resources Sampling Schedule Services Shipping Signal Transduction Site Structure Synaptic Vesicles Techniques Titan Training United States National Institutes of Health Universities Vermont Virginia Virus Virus Diseases Visit college contrast imaging cost data exchange detector experience faculty support flexibility high resolution imaging image processing improved insight instrument inter-institutional medical schools method development movie operation particle pathogenic bacteria portability protein complex public health relevance quantum reconstruction repaired supercomputer symposium three dimensional structure tomography tool transmission process

项目摘要

PROJECT SUMMARY Recently, single particle cryo-electron microscopy (cryo-EM) combined with 3-D reconstruction has emerged as a revolutionary tool for solving high-resolution 3-D structures of viruses and macromolecular complexes. The rapidly increasing number of near-atomic resolution (3-4Ǻ) structures solved using cryo-EM has allowed unprecedented atomic level understanding of fundamental cellular processes and viral infections. To obtain near-atomic resolution cryo-EM structures, requires the collection of high-resolution image data using state-of- the-art imaging resources, including both a high-end transmission electron microscope and a direct electron detector. The direct electron detectors not only improve image resolution and contrast, but also record movies for subsequent computational correction of electron beam-induced, sample movements during exposure. The increased image contrast and resolution are essential for solving near-atomic resolution structures of small protein complexes. However, the high cost to purchase and maintain a state-of-the-art cryo-EM resource, with both a high-end electron microscope and a direct electron detector, precludes many cryo-EM investigators from having access to these new techniques. Here, the creation of a Midwest Consortium for High- Resolution Cryo-electron Microscopy is proposed to provide access to such high-resolution data collection capability for cryo-EM laboratories without access to such resources. This consortium will consist of 4 investigators from the host institution (Purdue Univ.) which will maintain the high-resolution data collection resource (Titan Krios 300kV FEG microscope with phase plate, energy filter, and direct electron detector) and will provide services to 11 investigators from 10 partnering institutions (Boston Univ. School of Medicine, Michigan State Univ., Penn State College of Medicine, Rutgers Univ., Univ. of Alabama at Birmingham, Univ. of Colorado Boulder, Univ. of Kansas, Univ. of Missouri, Univ. of Vermont, and Virginia Commonwealth Univ.). The collective experience of the Purdue facility staff, faculty and onsite service engineer, in high-resolution cryo-EM imaging, will ensure that the facility operates at peak performance with minimal service interruptions. The high-resolution data collection capabilities established at Purdue and accessible to the partnering labs, will allow these investigators, who are all, except for the 3 new investigators, NIH-funded, to overcome the resolution barrier and facilitate discovery within their own cryo-EM projects on a range of structures, such as, bacterial pathogen adhesion proteins, human viruses, Huntington's Disease proteins, synaptic vesicle proteins, chemoreceptor signaling complex, etc. The Consortium will provide comprehensive support to the cryo-EM projects, including shipping samples, preparing sample grids, collecting high-resolution single particle and tomography images, processing raw movies, and transferring data back to the partners' labs. The data collection services will range from full data collection where partners simply mail in the samples and then receive the image data, to hands-on operations of the cryo-EM by partners trained by the host facility's staff.

项目总结

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Integrating on-grid immunogold labeling and cryo-electron tomography to reveal photosystem II structure and spatial distribution in thylakoid membranes.

DOI：
10.1016/j.jsb.2021.107746
发表时间：
2021-09
期刊：
Journal of structural biology
影响因子：
3
作者：
Jiang J;Cheong KY;Falkowski PG;Dai W
通讯作者：
Dai W

Cryo-EM structures and functional characterization of homo- and heteropolymers of human ferritin variants.

DOI：
10.1038/s41598-020-77717-4
发表时间：
2020-11-26
期刊：
Scientific reports
影响因子：
4.6
作者：
Irimia-Dominguez J;Sun C;Li K;Muhoberac BB;Hallinan GI;Garringer HJ;Ghetti B;Jiang W;Vidal R
通讯作者：
Vidal R

Architecture of a complete Bce-type antimicrobial peptide resistance module.