Cryoelectron Microscopy Equipment

这是一项建议，包括两项设备，一个低温工作台和一个低温传输系统，以完全装备低温电子显微镜系统。低温电子显微镜是一种观察无伪影生物材料的技术。这项技术是将标本冷冻在低温的水中，然后在零下140摄氏度的情况下用电子显微镜观察标本。研究人员将使用该设备来观察脂质体悬浮液、病毒、细胞器和膜泡。此外，还将使用未经化学固定的样品的薄冰冻切片来检查使用其他技术进行的观测的保真度。

This is a proposal for two items of equipment, a cryostage and a cryotransfer system to fully equip a low temperature electron microscope system. Cryoelectron microscopy is a technique for viewing artefact-free biological material. The technique is to preserve the specimen by freezing in water at low temperatures and then viewing the specimen with the electron microscope at -140o C. The equipment will be used by the investigators to view liposome suspensions, viruses, and cell organelle and membrane vesicles. In additions, thin frozen sections of chemically unfixed specimens will be used to check the fidelity of observations made using other techniques.