APTAMERS TO ANALYZE REGULATORY NETWORKS IN DROSOPHILA

这个项目的目标是开发多肽异构体的用途。 干扰体内蛋白质功能的物质。多肽异构体很短 相互作用的多肽(融合到平台分子E.Coli硫氧还蛋白) 特别是与目标蛋白结合。干扰特定蛋白质的异构体/ 可以使用酵母双杂交系统选择蛋白质相互作用，然后 在果蝇中以空间和时间控制的模式表达。 该小组以前的研究结果表明，可以使用多肽异构体。 在体内特异性靶向和抑制细胞周期调节因子，并使用两种 混合方法他们已经确定了一个蛋白质相互作用网络， 包括已知和潜在的细胞周期调节因子。该提案有四个方面 明确的目标。1)改进分离和亚克隆技术 顾客。2)通过表达干扰端粒的端粒来测试端粒方法 CDK2、Cyclin E和Dacapo在果蝇眼中的相互作用3)至 部分表征来自相互作用蛋白网络的新蛋白 找出一个可能调节细胞周期的核心组 发育中的眼睛。4)利用多肽异构体分析该核心的功能。 一组蛋白质。

The goal of this project is to develop the use of peptide aptomers that interfere with protein function in vivo. Peptide aptomers are short peptides (fused to a platform molecule, E. coli thioredoxin) that interact specifically with a target protein. Aptomers that disrupt specific protein/ protein interactions can be selected using the yeast two-hybrid system and then expressed in Drosophila in spatially and temporally controlled patterns. Previous results from this group have shown that peptide aptomers can be used to specifically target and inhibit cell cycle regulators in vivo, and using two hybrid approaches they have identified a network of protein interactors, that includes known and potential cell cycle regulators. The proposal has four Specific Aims. 1) To improve the technology for the isolation and subcloning of aptomers. 2) To test the aptomer approach by expressing aptomers that disrupt interactions between Cdk2, Cyclin E and Dacapo in the Drosophila eye. 3) To partially characterize novel proteins from a network of interacting proteins to identify a core group that are likely to regulate the cell cycle in the developing eye. 4) To use peptide aptomers to analyze the function of this core group of proteins.