Gene Suppression: Drug Target Validation

描述（由申请人提供）：人们对应用不同的化学和生物学工具来控制特定基因的表达或损害特定mRNA和蛋白质的活性非常感兴趣。生物技术行业为植物和动物工程开发了这些工具，制药行业利用这些工具来验证药物靶点，医学界研究了这些试剂的一个子集作为自己的治疗方法。本次Keystone研讨会的目的是回顾化学和生物学方法在转录，转录后和蛋白质水平上选择性关闭基因活性的实用性-特别是参考功能基因组学。其目的是提供一个广泛的概述，建立和新兴的方法，这些方法背后的基础科学和它们的效用从工业和医疗的角度来看。人类基因组的存在和微阵列战略在评估特异性方面的广泛使用，应使这次会议特别及时，并在2003年之前提供一个统一的重点。本文将讨论RNA干扰、抑制转录的蛋白质工程、基因控制的化学方法、反义、核酶和基因敲除策略等方面的最新进展。

DESCRIPTION (provided by applicant): There is an enormous interest in the application of diverse chemical and biological tools to control the expression of specific genes or compromise the activities of specific mRNAs and proteins. The biotechnology industry develops these tools for plant and animal engineering, the pharmaceutical industry makes use of these tools to validate drug targets, and the medical community investigates a subset of these reagents as therapeutics in their own right. The purpose of this Keystone Symposium is to review the utility of chemical and biological approaches to selectively turning gene activity off at the transcriptional, post-transcriptional and protein level- especially with reference to functional genomics. The aim is to provide a broad overview of established and emerging methodologies, the basic science behind these approaches and their utility from an industrial and medical perspective. The availability of the human genome and widespread use of micro array strategies for assessing specificity should make the meeting especially timely and provide a unifying focus by 2003. The latest advances on RNA interference, protein engineering to repress transcription, chemical approaches to gene control, antisense, ribozymes and knockout strategies will be discussed.