Toll Signaling Pathway in the Zebrafish

描述(由申请人提供)：这项建议的重点是确定Toll信号转导途径与斑马鱼先天免疫反应之间的关系。通过研究Toll通路对传染病的反应方式，并通过加深对先天免疫这一方面如何调节适应性免疫的理解，我们寻求找到增强、延伸和延长免疫反应的方法。 我们将对Toll途径进行功能分析，并开发将成为该领域其他研究人员必不可少的工具的试剂。这项建议的具体目的是： 1.确定Toll信号通路在多大程度上与先天免疫反应的激活有关。应用核因子-kappaB指示性转基因FISH评价Toll通路在感染应答中的作用 通过研究斑马鱼中的Toll信号通路并确定其在病原体保护性免疫中的作用，我们将更全面地了解天然免疫对免疫系统功能和发育的影响，以及这一途径如何促进现代适应性免疫反应的进化。

DESCRIPTION (provided by the applicant): The focus of this proposal is to define the relationship between the Toll signal transduction pathway and the innate immune response of the zebrafish. By examining the way in which the Toll pathway responds to infectious diseases, and by developing a deeper understanding of how this facet of innate immunity regulates adaptive immunity, we seek to find ways to augment, extend, and prolong the immune response. We will conduct functional analysis of the Toll pathway and develop reagents that will be essential tools for other researchers in this field. The SPECIFIC AIMS of this proposal are to: 1. Determine the extent to which the Toll signaling pathway is linked to the activation of the innate immune response. Evaluate the role of the Toll pathway in response to infection using NF-kappaB indicator transgenic fish By examining the Toll signaling pathway in the zebrafish and determining its role in protective immunity against pathogens, we will gain a more complete understanding of the impact of innate immunity on the function and development of the immune system, and how this pathway has contributed to the evolution of the modern adaptive immune response.