Dissecting the interplay between cardiomyocytes and fibroblasts using human stem cell models of disease

项目目标：1.建立和优化人多能干细胞-心肌细胞(hPSC-CM)和心脏成纤维细胞2D共培养和高分辨电生理表型的方法学；2.研究心肌细胞-成纤维细胞偶联对电功能和钙离子转运的影响；3.利用Cas9/CRISPR技术建立表达常见肌节基因突变的hPSC-CM系，并评价其对心肌细胞-成纤维细胞共培养中电生理和线粒体功能的影响。

Project aims: 1. Develop and optimise methodology for 2D co-culture and high resolution electrophysiological phenotyping of human pluripotent stem cell-cardiomyocytes (hPSC-CM) and cardiac fibroblasts, 2. Characterise the effect of cardiomyocyte-fibroblast coupling on electrical function and calcium handling, 3. Utilise Cas9/CRISPR technology to generate hPSC-CM lines expressing common sarcomeric gene mutations and asses their effects on electrophysiological and mitochondrial function in cardiomyocyte-fibroblast co-cultures.