From metabolic markers to mechanism of growth suppression: understanding the molecular physiology of sprouting in potato

对于价值40亿英镑的英国马铃薯产业来说，在储存期间控制发芽至关重要，但越来越多的法律限制对化学抑制剂构成了严峻的挑战，最广泛使用的农用化学品可能会被禁止。要找到解决问题的新方法，需要对发芽过程有更深入的了解。发芽涉及从块茎“眼”中静止的干细胞群重新激活和从周围块茎中动员储存储备。通过对分阶段解剖芽的代谢组学研究，我们发现TCA循环代谢物是发芽的早期标志。TCA通路是与ATP生成相关的核心过程。然而，有重要证据表明，在某些条件下，该途径也可以在特定组织中以局部、非循环的方式起作用，在碳骨架的产生和降低生长功率方面发挥重要作用。我们的数据与假设是一致的，即在萌芽的最初阶段，TCA“循环”是以这种非规范的方式工作的。了解芽发芽周围tca相关事件的序列和空间模式

Controlling sprouting during storage is critical to the £4bn UK potato industry yetincreasing legislative restictions on chemical suppressants pose seriouschallenges, with a potential ban on the most widely used agrichemical. A deeperunderstanding of the sprouting process is required to identify novel solutions tothe problem.Sprouting involves re-activation from quiescence of groups of stem cells in tuber"eyes" and mobilisation of storage reserves from the surrounding tuber. Via ametabolomic study on staged, dissected buds we have shown that TCA cyclemetabolites are very early markers of sprouting.The TCA pathway is a core process generally linked with ATP generation.However, there is significant evidence that the pathway can also work in apartial, non-cyclic fashion in specific tissues under certain conditions, playing animportant role in the generation of carbon skeletons and reducing power forgrowth. Our data are consistent with the hypothesis that during the earlieststages of sprouting the TCA "cycle" is working in such a non-canonical fashion.Understanding the sequence and spatial patterning of TCA-related eventsaround the bud sprouting