DELIVERY OF ANTIOXIDANT ENZYMES & GENES TO NEONATAL LUNG

描述：(改编自《调查者摘要》)：目标1将决定 保护细胞免受伤害所需的补充AOE的最佳组合 高氧症。这将使用肺上皮细胞培养系统来完成。 暴露在高氧环境中。目标2是改善AOE在体内的分布和吸收 肺部。稳定的悬浮液将在细胞培养和小鼠身上进行测试 暴露在高氧中，因为它们对细胞活力，病毒感染性， 多基因的传递和表达，以及损伤的程度。AIM 3将使用 人SP-B基因控制下含AOE基因的病毒载体 启动子(靶向II型或Clara细胞)或CMV启动子。目标4是 比较PFC蛋白和腺病毒相关的AOE悬液对小鼠血管内皮细胞生长的影响 正常或受伤，早产或足月羔羊肺，使用水平和 转基因表达的持续时间。

DESCRIPTION: (Adapted from the Investigator's Abstract): Aim 1 will determine the optimal combination of supplemental AOE required to protect cells from hyperoxia. This will be done using a pulmonary epithelial cell culture system exposed to hyperoxia. Aim 2 is to improve the distribution and uptake of AOE in the lung. Stabilized suspensions will be tested in cell culture and in mice exposed to hyperoxia for their effects on cell viability, virus infectivity, multiple gene delivery and expression, and degree of injury. Aim 3 will use viral constructs containing AOE genes under the control of the human SP-B promoter (to target Type II or Clara cells) or the CMV promoter. Aim 4 is to compare the effects of PFC-protein or adenoviral-associated AOE suspensions on normal or injured, preterm or term lamb lungs, using assays for levels and duration of transgene expression.