Oral spirochetes: molecular genetic analysis

描述：这个应用程序将继续检查分子基础 螺旋体的毒性。利用构建的齿密螺旋体突变体 在之前的赠款期间，运动性、趋化性和 将对生物膜形成过程中的蛋白酶活性进行研究。在……里面 此外，将使用RNA差异显示方法来识别基因， 它们参与了固体表面的定植，预制的生物膜 牙龈卟啉单胞菌和核梭杆菌，以及宿主细胞表面。 这些方法将有助于确定口服螺旋体的机制 用于定植于龈下缘部。此外，由于渗透了 螺旋体的组织屏障似乎是一种重要的致病因子 这些生物体，这一特性的分子基础将在T 丹蒂科拉。利用HUVEC细胞转运系统，螺旋体的基因， 都参与了组织渗透，将通过基因识别 接近了。然后将有兴趣确定梅毒微生物是否 梅毒螺旋体利用一组同源基因入侵组织。此外，如果 后一种生物利用物种特有的入侵基因，假设 与梅毒螺旋体有关的口腔螺旋体可能在 将检查牙周炎。这些方法应该会增加理解 螺旋体致病性的分子基础。

DESCRIPTION: This application will continue to examine the molecular basis for the virulence of spirochetes. Utilizing Treponema denticola mutants constructed in the previous grant period, the respective roles of motility, chemotaxis, and protease activity in the formation of biofilms will be investigated. In addition, a RNA differential display approach will be used to identify genes, which are involved in colonization of solid surfaces, preformed biofilms of Porphyromonas gingivalis and Fusobacterium nucleatum, and host cell surfaces. These approaches will help to define the mechanisms, which oral spirochetes utilize in colonizing the subgingival margin. Furthermore, since penetration of tissue barriers by spirochetes appears to be an important virulence factor of these organisms, the molecular basis for this property will be examined in T denticola. Using the HUVEC transcytosis system, genes of the spirochete, which are involved in tissue penetration, will be identified using genetic approaches. It will then be of interest to determine if the syphilitic organism T pallidum utilizes a homologous set of genes to invade tissue. In addition, if the later organism utilizes species-specific invasion genes, the hypothesis that oral spirochetes, which are related to T. pallidum, may play a role in periodontitis will be examined. These approaches should increase understanding of the molecular basis for the pathogenicity of spirochetes.