Molecular Biology of Eukaryotic Viruses

描述(由申请人提供)：本提案是为继续支持1988年在加州大学欧文分校(UCI)建立的真核病毒分子生物学研究培训计划而提出的竞争性续签申请。这个项目的目标是在分子病毒学的基本方面培训博士研究生，因为分子病毒学与基因表达调控、病毒结构、病毒-宿主相互作用和致病机制有关。在目前的审查期间，我们扩大了该计划，将重点放在病毒蛋白质组学的跨学科培训中，因为它与病毒蛋白质的结构以及完整的病毒颗粒有关。在过去的颁奖周期中(9/98-8/03)，11名博士前学生得到了该培训计划的支持。参与该项目的教职员工包括来自四个系的十名教职员工。这些系的教员与生物科学学院和医学院其他系的教员一起参加UCI分子生物学、遗传学和生物化学研究生项目(MBG&B)，该项目负责博士前学生的招生、录取和初步培训。所有NIH病毒学助学金学员都属于MBG&B项目中的病毒学专业。这一轨道由在病毒学和相关学科中有共同研究兴趣的教职员工、学生、博士后研究员和实验室工作人员组成。病毒学课程的培训计划包括病毒基因表达、病毒感染的分子发病机制和疾病的免疫致病机制等选修课。教员的研究项目包括病毒基因组复制、病毒特异性转录、RNA加工、病毒翻译和蛋白质加工、病毒结构蛋白的组装和运输以及病毒颗粒的结构。病毒学教授还在研究病毒与宿主的相互作用，包括宿主调控分子的改变、生长控制、细胞周期调控、分化调控、病毒基因组整合到宿主细胞DNA的机制以及宿主功能对病毒基因表达的颠覆。正在研究的病毒/病毒系统包括小鼠白血病病毒和绵羊逆转录病毒、艾滋病毒、酵母Ty3、脊髓灰质炎病毒和人鼻病毒、冠状病毒、单纯疱疹病毒、人乳头瘤病毒、多瘤病毒、腺病毒和一些含有RNA的植物病毒。

DESCRIPTION (provided by applicant): This proposal is a competing renewal application for the continuing support of a training program for the study of the Molecular Biology of Eukaryotic Viruses established at the University of California, Irvine (UCI) in 1988. The goal of this program is to train Ph.D. graduate students in the fundamental aspects of molecular virology as they relate to the regulation of gene expression, virus structure, virus-host interactions, and pathogenesis. During the current review period, we expanded the program to include an emphasis in interdisciplinary training in viral proteomics as it relates to structures of viral proteins as well as intact virus particles. During the past award cycle (9/98-8/03), eleven predoctoral students were supported by this training program. The participating faculty for this program include ten faculty members from four departments. Faculty members from these departments, along with those from other departments in the School of Biological Sciences and the College of Medicine, participate in the Graduate Program in Molecular Biology, Genetics, & Biochemistry (MBG&B) at UCI, which oversees recruitment, admission, and initial training of predoctoral students. All of the NIH Virology Grant trainees belong to the Virology Track in the MBG&B program. This track is comprised of faculty, students, postdoctoral fellows, and laboratory staff who have shared research interests in virology and related disciplines. The training program for the Virology Track includes elective courses in viral gene expression, molecular pathogenesis of viral infections, and immunopathogenic mechanisms of disease. The research programs of faculty participants include the study of viral genome replication, viral-specific transcription, RNA processing, viral translation and protein processing, assembly and transport of viral structural proteins, and the structures of virus particles. The virology faculty are also studying virus-host interactions that include alteration of host regulatory molecules, growth control, cell cycle regulation, differentiation control, the mechanisms of integration of viral genomes into host cell DNA, and the subversion of host functions for virus gene expression. The viruses/viral systems being studied include murine leukemia virus and a sheep retrovirus, HIV, yeast Ty3, poliovirus and human rhinovirus, coronaviruses, herpes simplex virus, human papillomavirus, polyomavirus, adenovirus, and a number of RNA-containing plant viruses.