Molecular Biology of Eukaryotic Viruses

描述（由申请人提供）：本提案是一项竞争性续期申请，旨在继续支持加州大学欧文分校（UCI）于1988年建立的真核病毒分子生物学研究培训计划。该计划的目标是培养博士。研究生在分子病毒学的基本方面，因为他们涉及到基因表达，病毒结构，病毒宿主相互作用的调节，和发病机制。在目前的审查期间，我们扩大了该计划，包括在病毒蛋白质组学的跨学科培训的重点，因为它涉及到病毒蛋白质的结构以及完整的病毒颗粒。在过去的奖励周期（9/98-8/03），11名博士前的学生支持这个培训计划。参加该计划的教师包括来自四个部门的十名教师。来自这些部门的教职员工，沿着来自生物科学学院和医学院其他部门的教职员工，参加UCI的分子生物学，遗传学和生物化学研究生课程（MBG&B），该课程负责招聘，录取和博士前学生的初步培训。所有NIH病毒学资助的受训人员都属于MBG&B计划的病毒学轨道。这条赛道由教师，学生，博士后研究员和实验室工作人员组成，他们在病毒学和相关学科中有共同的研究兴趣。病毒学轨道的培训计划包括病毒基因表达，病毒感染的分子发病机制和疾病的免疫病理机制的选修课程。教师参与的研究项目包括病毒基因组复制，病毒特异性转录，RNA加工，病毒翻译和蛋白质加工，病毒结构蛋白的组装和运输以及病毒颗粒的结构的研究。病毒学系也在研究病毒与宿主的相互作用，包括宿主调节分子的改变、生长控制、细胞周期调节、分化控制、病毒基因组整合到宿主细胞DNA中的机制，以及病毒基因表达的宿主功能的颠覆。正在研究的病毒/病毒系统包括鼠白血病病毒和绵羊逆转录病毒、HIV、酵母Ty 3、脊髓灰质炎病毒和人鼻病毒、冠状病毒、单纯疱疹病毒、人乳头瘤病毒、多瘤病毒、腺病毒和许多含RNA的植物病毒。

DESCRIPTION (provided by applicant): This proposal is a competing renewal application for the continuing support of a training program for the study of the Molecular Biology of Eukaryotic Viruses established at the University of California, Irvine (UCI) in 1988. The goal of this program is to train Ph.D. graduate students in the fundamental aspects of molecular virology as they relate to the regulation of gene expression, virus structure, virus-host interactions, and pathogenesis. During the current review period, we expanded the program to include an emphasis in interdisciplinary training in viral proteomics as it relates to structures of viral proteins as well as intact virus particles. During the past award cycle (9/98-8/03), eleven predoctoral students were supported by this training program. The participating faculty for this program include ten faculty members from four departments. Faculty members from these departments, along with those from other departments in the School of Biological Sciences and the College of Medicine, participate in the Graduate Program in Molecular Biology, Genetics, & Biochemistry (MBG&B) at UCI, which oversees recruitment, admission, and initial training of predoctoral students. All of the NIH Virology Grant trainees belong to the Virology Track in the MBG&B program. This track is comprised of faculty, students, postdoctoral fellows, and laboratory staff who have shared research interests in virology and related disciplines. The training program for the Virology Track includes elective courses in viral gene expression, molecular pathogenesis of viral infections, and immunopathogenic mechanisms of disease. The research programs of faculty participants include the study of viral genome replication, viral-specific transcription, RNA processing, viral translation and protein processing, assembly and transport of viral structural proteins, and the structures of virus particles. The virology faculty are also studying virus-host interactions that include alteration of host regulatory molecules, growth control, cell cycle regulation, differentiation control, the mechanisms of integration of viral genomes into host cell DNA, and the subversion of host functions for virus gene expression. The viruses/viral systems being studied include murine leukemia virus and a sheep retrovirus, HIV, yeast Ty3, poliovirus and human rhinovirus, coronaviruses, herpes simplex virus, human papillomavirus, polyomavirus, adenovirus, and a number of RNA-containing plant viruses.