Core--Animal Resources

具体目标： 这个核心单元的总体目标是协调本计划项目五个项目中每一个项目的调查人员所需的动物的使用。为实现这一职能，核心将实现以下具体目标。 特异性目的1是维持(MRen2)2 7转基因(TG)高血压大鼠、新培育的同源mRen2 Lewis大鼠、血管紧张素原反义转基因[ASrAogen TG]大鼠、汉诺威SpragueDawley(Hann SD)大鼠的育种群体，建立ACE2基因敲除(ACE2 KO)小鼠繁育群体。 特异性AIM-2是根据五个项目的科学目标，分别产生半合子(MRen2)TG和mRen2。Lewis大鼠、纯合子ASrAogen大鼠、汉诺威SD大鼠和ACE2KO小鼠。此外，核心股将监督从商业供应商采购老鼠和老鼠。 具体目标3将是协调维克森林大学医学院动物资源计划(ARP)为本计划项目拨款研究人员使用的所有动物提供的住房。 具体的目标4将是协助研究人员为本计划项目拨款中提议的实验准备动物。特别是，作为核心领导者，Averill博士将在大鼠和小鼠用于长期测量动脉血压和血流的仪器方面提供技术建议和协助。

Specific Aims: The overall goal of this Core Unit is to coordinate the use of animals needed by investigators of each of the five projects of this Program Project. To accomplish this function, the Core will implement the following Specific Aims. SPECIFIC AIM 1 is to maintain breeder colonies of (mRen2)27 transgenic (TG) hypertensive rats, the newly developed congenic mRen2.Lewis rats, angiotensinogen antisense transgenic [ASrAogen TG] rats, Hannover Sprague-Dawley (Hann SD) rats, and to establish a breeding colony of ACE2 knockout (ACE2 KO) mice. SPECIFIC AIM 2 is to produce hemizygous (mRen2) TG and mRen2.Lewis rats, homozygous ASrAogen rats, Hannover SD rats, and ACE2 KO mice as appropriate to the scientific objectives in each of the five projects. In addition, the Core Unit will oversee the procurement of rats and mice from commercial vendors. SPECIFIC AIM 3 will be to coordinate the housing provided by the Animal Resources Program (ARP) at the Wake Forest University School of Medicine for all animals used by investigators of this Program Project grant. SPECIFIC AIM 4 will be to assist investigators in the preparation of animals for experiments proposed in this Program Project grant. In particular, Dr. Averill, as the Core Leader, will provide technical advice and assistance in the instrumentation of rats and mice for chronic measurement of arterial blood pressure and blood flow.