Core--Animal Resources

核心--动物资源

• 批准号: 7586117
• 负责人: DAVID B. AVERILL
• 金额: $ 19.15万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7586117
• 关键词: Angiotensinogen; Animal Experiments; Animals; Blood Pressure; Blood flow; Breeding; Chronic; Goals; Housing; Knockout Mice; Measurement; Mus; Preparation; Program Research Project Grants; Rattus; Research Personnel; Sprague-Dawley Rats; Transgenic Organisms; Universities; Vendor; animal resource; congenic; forest; instrumentation; medical schools; programs

Specific Aims: The overall goal of this Core Unit is to coordinate the use of animals needed by investigators of each of the five projects of this Program Project. To accomplish this function, the Core will implement the following Specific Aims. SPECIFIC AIM 1 is to maintain breeder colonies of (mRen2)27 transgenic (TG) hypertensive rats, the newly developed congenic mRen2.Lewis rats, angiotensinogen antisense transgenic [ASrAogen TG] rats, Hannover Sprague-Dawley (Hann SD) rats, and to establish a breeding colony of ACE2 knockout (ACE2 KO) mice. SPECIFIC AIM 2 is to produce hemizygous (mRen2) TG and mRen2.Lewis rats, homozygous ASrAogen rats, Hannover SD rats, and ACE2 KO mice as appropriate to the scientific objectives in each of the five projects. In addition, the Core Unit will oversee the procurement of rats and mice from commercial vendors. SPECIFIC AIM 3 will be to coordinate the housing provided by the Animal Resources Program (ARP) at the Wake Forest University School of Medicine for all animals used by investigators of this Program Project grant. SPECIFIC AIM 4 will be to assist investigators in the preparation of animals for experiments proposed in this Program Project grant. In particular, Dr. Averill, as the Core Leader, will provide technical advice and assistance in the instrumentation of rats and mice for chronic measurement of arterial blood pressure and blood flow.

具体目标： 该核心单位的总体目标是协调本计划项目的五个项目中每个项目的研究者所需的动物使用。为实现这一职能，核心将实现以下具体目标。 具体目的1是维持（mRen 2）27转基因（TG）高血压大鼠、新开发的同类mRen2.Lewis大鼠、血管紧张素原反义转基因[ASrAogen TG]大鼠、汉诺威Sprague-Dawley（Hann SD）大鼠的繁殖群体，并建立ACE 2敲除（ACE 2 KO）小鼠的繁殖群体。 具体目标2是根据5个项目中的科学目标，生产半合子（mRen 2）TG和mRen2.Lewis大鼠、纯合子ASrAogen大鼠、汉诺威SD大鼠和ACE 2 KO小鼠。此外，核心股将监督从商业供应商采购大鼠和小鼠的工作。 具体目标3将是协调由动物资源计划（阿普）在维克森林大学医学院提供的住房，用于本计划项目赠款的研究人员使用的所有动物。 具体目标4将协助研究人员准备动物的实验中提出的这个计划项目赠款。特别是，Averill博士作为核心负责人，将在大鼠和小鼠的仪器方面提供技术咨询和帮助，以长期测量动脉血压和血流量。