Laboratory Core

STD-TM-CRC实验室核心将1)为在STD-TMCRC内执行临床、流行病学和行为项目的调查人员提供必要的专业微生物学和实验室专业知识；2)促进基础科学或分子生物学方面的新进展迅速应用于STD-TM-CRC内的相关实验室和流行病学项目；3)为其他研究性传播疾病的研究人员提供国家和国际参考实验室；以及4)维持衣原体和淋病登记册。核心中提供的实验室专门知识将包括高度专业化的实验室测试，这些测试即使在其他研究实验室中也不是常规可用的，但对于拟议的流行病学或临床研究的成功是不可或缺的。这类测试的例子包括沙眼衣原体血清型的测定；淋球菌缺陷型和血清型的测定；核酸扩增试验 衣原体、淋病和生殖支原体；淋球菌的脉冲场凝胶电泳(PFGE)分析；选定衣原体菌株的MOMP核苷酸序列；沙眼衣原体的微型IF血清学。在核心实验室提供这些测试和其他测试确保了这些测试将由技术经验丰富的研究人员进行，并通过规模经济降低了成本，并使进行行为和流行病学研究的研究人员可以使用这些测试，否则他们可能无法接触到实验室合作者。该核心还将积极寻求将适当的技术从基础科学实验室转移到相关的临床和流行病学项目。将根据需要制定和实施新的程序。这一资助期的例子将包括滴虫和生殖支原体的核酸扩增测试以及使用尿样检测HPV-DNA。在实验室核心内进行的程序将作为国家和国际资源提供给其他研究人员，使他们不需要开发昂贵和技术要求高的测试。

The STD-TM-CRC laboratory core will 1) provide necessary specialized microbiological and laboratory expertise for investigators performing clinical, epidemiological and behavioral projects within the STD-TMCRC; 2) facilitate the rapid application of new advances in basic sciences or molecular biology to relevant laboratory and epidemiological projects within the STD-TM-CRC; 3) serve as a national and international reference laboratory for other investigators studying STDs; and 4) maintain a chlamydia and gonorrhea registry. The laboratory expertise provided in the core will consist of highly specialized laboratory tests that are not routinely available even in other research laboratories but are integral to the success of proposed epidemiological or clinical research. Examples of such tests include determination of C. trachomatis serovars; determination of gonococcal auxotypes and serotypes; nucleic acid amplification tests for chlamydia, gonorrhea and Mycoplasma genitalium; pulse field gel electrophoresis (PFGE) analysis of N. gonorrhoeae; MOMP nucleotide sequences of selected chlamydia strains; and micro IF serology for C. trachomatis. Provision of these and other tests in a core laboratory ensures that they will be performed by technically experienced investigators, reduces costs by economy of scale, and makes these tests available to researchers conducting behavioral and epidemiological studies who might otherwise not have access to laboratory collaborators. The core will also actively seek to transfer appropriate technology from basic science laboratories to relevant clinical and epidemiological projects. New procedures will be developed and implemented as needed. Examples in this funding period will include nucleic acid amplification tests for trichomonas and M. genitalium and detection of HPV-DNA using urine specimens.The procedures performed within the laboratory core will be available as a national and international resource for other investigators, eliminating the need for them to develop expensive and technically demanding tests.