CORE--LABORATORY FACILITY

核心--实验室设施

• 批准号: 6299634
• 负责人: WALTER E STAMM
• 金额: $ 15.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2000-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299634
• 关键词: Chlamydia trachomatis; Neisseria gonorrhoeae; biomedical facility; clinical research; communicable disease diagnosis; cooperative study; epidemiology; genetic strain; human data; human tissue; nucleic acid sequence; patient /disease registry; polymerase chain reaction; pulsed field gel electrophoresis; serotyping; sexually transmitted diseases

The STD-CRC laboratory core will: 1) provide necessary specialized microbiological and laboratory expertise for investigators performing clinical, epidemiological and behavioral projects within the STD-CRC; 2) facilitate the rapid application of new advances in basic sciences or molecular biology to relevant clinical and epidemiologic projects within the STD-CRC; 3) serve as a national and international reference laboratory for other investigators studying STDs; and 4) maintain a gonorrhea registry to continue assessment of factors associated with reinfection with Neisseria gonorrhoeae. The laboratory expertise provided in the core will consist of highly specialized laboratory tests that are not routinely available even in other research laboratories but are integral to the success of proposed epidemiological or clinical research. Examples of such tests include determination of C. trachomatis serovars; determination of gonococcal auxotypes and serotypes; PCR and LCR tests for chlamydia; Pulse field gel electrophoresis (PFGE) analysis of N. gonorrhoeae; MOMP nucleotide sequences of selected chlamydia strains; and Micro IF serology for C. trachomatis. Provision of these and other tests in a central core laboratory ensures that they will be performed by technically experienced investigators, reduces costs by economy of scale, and makes these tests available to researchers conducting behavioral and epidemiological studies who might otherwise not have access to laboratory collaborators. Such availability will foster the use of microbiological endpoints in behavioral studies. While it is not the purpose of the laboratory core to support basic science work, the core will actively seek to transfer appropriate technology from basic science laboratories to relevant clinical and epidemiological projects. For example, we propose the further development and implementation of a PFGE based typing system for gonococci that will demonstrate heterogeneity even among strains of the same auxotype and serovar, providing a much more powerful tool for demonstrating strain diversity. The procedures performed within the laboratory core will be available as a national and international resource for other investigators, eliminating the need for them to develop expensive and technically demanding tests.

STD-CRC实验室核心将：1）提供必要的专业 微生物和实验室专业知识， 在性病-CRC范围内的临床、流行病学和行为项目; 2） 促进基础科学新进展的快速应用， 分子生物学相关的临床和流行病学项目， 《性传播疾病和儿童权利公约》; 3）作为国家和国际参考 其他研究性病的研究人员的实验室;以及4）维持一个 淋病登记处继续评估与淋病相关的因素 淋病奈瑟菌再次感染提供的实验室专业知识 将由高度专业化的实验室测试组成， 即使在其他研究实验室也无法常规获得， 对拟议的流行病学或临床研究的成功不可或缺。 此类测试的实例包括C的测定。沙眼血清型; 淋球菌生长型和血清型的测定.聚合酶链反应和LCR试验 脉冲场凝胶电泳（PFGE）分析衣原体; 选择的衣原体菌株的MOMP核苷酸序列;和 C.的微量IF血清学。沙眼提供这些和其他测试 在一个中心核心实验室，确保他们将进行 技术经验丰富的调查人员，通过规模经济降低成本， 并将这些测试提供给研究人员， 流行病学研究，否则可能无法获得实验室 合作者这种可用性将促进微生物的使用， 行为研究的终点。 虽然实验室核心的目的不是支持基本的 科学工作，核心将积极寻求适当转移 从基础科学实验室到相关临床和 流行病学项目。例如，我们建议进一步发展 并实施基于PFGE的淋球菌分型系统， 甚至在相同营养型的菌株之间也表现出异质性， 血清型，提供了一个更强大的工具， 多样性在实验室核心内进行的程序将 作为国家和国际资源， 调查人员，消除了他们开发昂贵和 技术上要求很高的测试。