CORE--LABORATORY FACILITY

STD-CRC实验室核心将：(1)为在STD-CRC内开展临床、流行病学和行为项目的研究人员提供必要的专业微生物学和实验室专业知识；2)促进基础科学或分子生物学方面的新进展在STD-CRC内的相关临床和流行病学项目中的快速应用；3)为其他研究性病的研究人员提供国内和国际参考实验室；以及4)维持衣原体和淋病登记册，以继续评估与再感染相关的因素。核心中提供的实验室专门知识将包括高度专业化的实验室测试，这些测试即使在其他研究实验室中也不是常规可用的，但对于拟议的流行病学或临床研究的成功是不可或缺的。这些测试的例子包括：沙眼衣原体血清型的测定；淋球菌的缺陷型和血清型的测定；衣原体的PCR和LCR试验；淋球菌的脉冲场凝胶电泳(PFGE)分析；选定的衣原体菌株的MOMP核苷酸序列；沙眼衣原体的微量IF血清学和尿样上的HPV型特异性聚合酶链式反应。在中央核心实验室提供这些测试和其他测试确保了这些测试将由技术经验丰富的研究人员进行，并通过规模经济降低了成本，并使进行行为和流行病学研究的研究人员可以使用这些测试，否则他们可能无法联系到实验室合作者。这种可获得性将促进在行为研究中使用微生物终点。虽然实验室核心的目的不是支持基础科学工作，但核心将积极寻求将适当的技术从基础科学实验室转移到相关的临床和流行病学项目。例如，我们建议进一步开发和实施一种特定类型的HPV聚合酶链式反应，它将允许从尿样中进行诊断，从而促进基于人群的研究。在实验室核心内进行的程序将作为国家和国际资源提供给其他调查人员，使他们不需要开发昂贵和技术要求高的测试。

The STD-CRC laboratory core will: l) provide necessary specialized microbiological and laboratory expertise for investigators performing clinical, epidemiological and behavioral projects within the STD-CRC; 2) facilitate the rapid application of new advances in basic sciences or molecular biology to relevant clinical and epidemiological projects within the SDC-CRC; 3) serve as a national and international reference laboratory for other investigators studying STDs; and 4) maintain a chlamydia and gonorrhea registry to continue assessment of factors associated with reinfection. The laboratory expertise provided in the core will consist of highly specialized laboratory tests that are not routinely available even in other research laboratories but are integral to the success of proposed epidemiological or clinical research. Examples of such tests include determination of C. trachomatis serovars; determination of gonococcal auxotypes and serotypes; PCR and LCR tests for chlamydia; pulse field gel electrophoresis (PFGE) analysis of N. gonorrhoeae; MOMP nucleotide sequences of selected chlamydia strains; Micro IF serology for C. trachomatis and type specific HPV PCR on urine specimens. Provision of these and other tests in a central core laboratory ensures that they will be performed by technically experienced investigators, reduces costs by economy of scale, and makes these tests available to researchers conducting behavioral and epidemiological studies who might otherwise not have access to laboratory collaborators. Such availability will foster the use of microbiological endpoints in behavioral studies. While it is not the purpose of the laboratory core to support basic science work, the core will actively seek to transfer appropriate technology from basic science laboratories to relevant clinical and epidemiological projects. For example, we propose the further development and implementation of a type specific HPV PCR that will allow diagnosis from urine specimens, thus facilitating population based studies. The procedures performed within the laboratory core will be available as a national and international resource for other investigators, eliminating the need for them to develop expensive and technically demanding tests.