Purchase of 900 MHz NMR Spectrometer

描述(申请人提供)：我们建议建立一个900 MHz的落基山地区核磁共振中心，为落基山和中部地区的大量大学提供服务。核磁共振中心将设在科罗拉多大学健康科学中心的菲茨西蒙斯新校区，将为蛋白质、核酸和碳水化合物结构和功能领域的核磁共振结构生物学家提供富有成效的服务。用户群由8个机构的19名研究人员组成。位于菲茨西蒙斯的新的基础研究大楼的空间是专门为900 MHz核磁共振的独特选址要求而设计的，该大楼的建设正在顺利进行。TROSY型实验的最新发展使得能够获得比以前可能的更大的大分子的极高分辨率的核磁共振谱。这些TROSY实验是在超高场下优化的，所要求的最先进的900 MHz核磁共振谱仪和低温探头将使用户能够利用这些强大的新技术。该中心的主要目标将是提供对900 MHz核磁共振独特功能的常规访问，以加快他们的生物和生物医学研究计划。除了在现场获取核磁共振数据外，经验丰富的用户还可以在设施工作人员的帮助下通过互联网获取900 MHz的核磁共振数据。核磁共振中心的第二个主要目标将是建立和维护一个基于网络的协作馆，以共享六个“重点领域”的信息和专业知识。这些重点领域是用户群体的强项，而Collaboratemal将为有经验的用户提供一个宝贵的机制，让他们与其他用户分享他们的专业知识。核磁共振中心和网络协作室最初的六个重点领域是：提高蛋白质的核磁共振研究的分辨率和灵敏度；大分子复合体的核磁共振研究；蛋白质和核酸动力学的研究；RNA和DNA的核磁共振研究的改进方法；膜蛋白质的核磁共振研究；以及通过在低粘度溶剂中使用反胶束来提高光谱分辨率。 提出了一个设计良好的管理该设施的组织计划，该计划仿照其他已建立的多用户核磁共振和同步加速器设施的计划。900兆赫核磁共振的时间将根据书面提案分配，提案审查委员会将对其进行审查。NIH资助的研究需要900 MHz核磁共振的高场强、高分辨率和独特的能力，这将是该中心的最高优先事项。

DESCRIPTION (provided by applicant): We propose to establish a Rocky Mountain Regional NMR Center with a 900 MHz NMR serving a large number of Universities in the Rocky Mountain and Central Regions. The NMR Center will be situated on the new Fitzsimons campus of the University of Colorado Health Sciences Center, and will serve a productive group of NMR structural biologists in the areas of protein, nucleic acid and carbohydrate structure and function. The user group consists of 19 researchers at 8 Institutions. Space in the new Basic Research Building at Fitzsimons has been specifically designed for the unique siting requirements of a 900 MHz NMR and construction of the building is well underway. Recent developments in TROSY-type experiments allow acquisition of extremely high resolution NMR spectra on much larger macromolecules than was previously possible. These TROSY experiments are optimized at ultra-high fields, and the requested state-of-the-art 900 MHz NMR spectrometer and cryoprobe will make it possible for the users to take advantage of these powerful new techniques. The primary goal of the Center will be to provide routine access to the unique capabilities of the 900 MHz NMR in order to accelerate their biological and biomedical research programs. In addition to acquiring their NMR data on-site, experienced users will be able to acquire 900 MHz NMR data over the Internet with the aid of facility staff. A second major goal of the NMR Center will be to establish and maintain a Web-based collaboratorium for sharing information and expertise in a set of six "focus areas." These focus areas are strengths of the user group and the collaboratorium will provide a valuable mechanism for experienced users to share their expertise with other users. The six initial focus areas for the NMR Center and Web collaboratorium are: Improved Resolution and Sensitivity for NMR Studies of Proteins; NMR Studies of Large Macromolecular Complexes; Studies of Protein and Nucleic Acid Dynamics; Improved Methods for NMR Studies of RNA and DNA; NMR Studies of Membrane Proteins; and Improved Spectral Resolution by Employing Reverse Micelles in Low Viscosity Solvents. A well designed organizational plan for administering the facility is presented which is modeled after plans at other established multi-user NMR and synchrotron facilities. Time on the 900 MHz NMR will be allocated based on written proposals which will be reviewed by a proposal review committee. NIH-funded research that requires the high field, high resolution, and unique capabilities of the 900 MHz NMR will be the highest priority for the center.