Cryoprobe for NMR structural studies

科罗拉多大学医学院将购买低温冷却1H/13C/15N三重共振探针（cryoprobe）和相关的低温系统，用于现有的瓦里安Inova 600 MHz核磁共振（NMR）光谱仪。该系统将在NCRR共享仪器拨款计划和UCHSC生物分子结构计划提供的机构资金的支持下购买。该冷冻探针将用于生物分子结构和分子相互作用的研究，作为正在进行的NIH资助的UCHSC研究项目的一部分。购买这种探针是为了满足UCHSC研究人员不断增长的需求，以增加核磁共振数据收集的通量，并使以前样品溶解度和/或可用性由于现有核磁共振技术的灵敏度有限而禁止结构分析的新项目的研究成为可能。需求增加

The University of Colorado School of Medicine will purchase a cryogenically cooled 1H/13C/15N triple resonance probe (cryoprobe) and associated cryogenic system for use with an existing Varian Inova 600 MHz Nuclear Magnetic Resonance (NMR) Spectrometer. The system will be purchased with support from the NCRR Shared Instrumentation Grant program and institutional funds provided by the UCHSC Program in Biomolecular Structure. This cryoprobe will be used in the study of biomolecular structures and molecular interactions as part of ongoing NIH funded research programs at UCHSC. Purchase of this probe is required to meet the growing demands of investigators at UCHSC to increase the throughput of NMR data collection and to enable the study of new projects where previously sample solubility and/or availability prohibited structural analysis because of the limiting sensitivity of existing NMR technology. The increased demand results from the continuing expansion of the faculty in several departments within the School of Medicine and from the establishment of an interdepartmental Program in Biomolecular Structure, from planned new recruitments, and increased number of collaborative structural biology projects. The requested cryoprobe will significantly improve the existing resources by increasing data collection capacity and accelerating research in the following projects: Structural analysis of complex oligosaccharides from human cell lines (Bendiak, B.) Structural studies on denatured states of proteins (Bowler, B.) Structural analysis of Dynein motor proteins and coiled-coils (Hodges R.) Molecular basis of alcohol's actions (Jones, D.) Structure and function of IRES RNA sequence in protein translation (Kieft, J.) Mechanism of the phospholipid-dependent signaling by Faciogenital Dysplasia 1 (Kutateladze, T.) Structural analysis of retrotransposition in LINE-1 (Martin, S.) Structure and function of membrane and protein targeting domains (Overduin, M.) Use of NMR spectroscopy in diagnosis of disease and drug toxicology (Serkova, N.)