Multi-Parameter Bench-top Flow Cytometer

描述（申请人提供）：流式细胞术是生物医学研究中细胞和亚细胞分析的强大技术。它的主要能力在于它能够快速地对单个细胞进行多参数分析，从而允许识别和分离重要的细胞子集。北卡罗来纳大学教堂山分校的流式细胞仪设备成立于1982年，旨在提供这项关键的研究核心服务。该设施是唯一提供研究流式细胞术服务的研究人员在UNC-CH。该设施目前有3台流式细胞仪（2台FACScans， 1台FACSCalibur）和1台分选仪（MoFIo）。这些仪器被大量使用（上一财年充电5174.5小时），每年为100多名调查人员服务。在本财政年度的前6个月（2002年7月1日至2002年12月31日），81名调查人员共被起诉2699小时。我们机构在传染病研究方面的一项新的重大倡议促使我们要求将现有的FACSCalibur转移到BSL-3设施，以便为传染病研究人员提供对活的传染病样本进行流式细胞术的能力。这将严重干扰我们一般使用的机器访问时间，并使我们无法进行越来越多的调查人员需要的四色分析。因此，在允许这一移动之前，我们必须替换FACSCalibur。此外，一些研究人员要求增加参数数量（5种以上颜色），更多和更低成本的Ca++动员实验，CFP的激发以及使用Hoechst或DAPI进行DNA含量分析。因此，为了满足这些日益增长的需求，取代FACSCalibur的四色能力和一般能力，并为未来的发展提供资金，我们正在申请购买一台四激光，多参数台式分析流式细胞仪。目前，我们认为来自DakoCytomation的CyAn最符合我们的要求，并要求，在此申请中，大部分资金用于购买仪器。除了所有安装/改装费用（9 500美元）外，北卡罗来纳大学医学院将提供65 500美元用于购买。学院还为流式细胞仪设备的运营提供了可观的持续支持——Uneberger综合癌症中心每年提供80,000美元，微生物和免疫学系提供设备主任工资的25%。

DESCRIPTION (provided by applicant): Flow cytometry is a powerful technology for cellular and subcellular analyses in biomedical research. Its primary power lies in its ability to rapidly perform multi-parametric analysis of individual cells, thereby permitting the identification and isolation of important subsets of cells. The Flow Cytometry Facility at The University of North Carolina at Chapel Hill was established in 1982 to provide this critical research core service. The Facility is the sole provider of research flow cytometry services to investigators at UNC-CH. The Facility currently has 3 analytical flow cytometers (2 FACScans, 1 FACSCalibur) and one sorter (MoFIo). The instruments are heavily used (last fiscal year 5,174.5 hours charged) and annually serve over 100 investigators. During the first 6 months of this fiscal year (July 1,2002 to Dec. 31, 2002) 2,699 hours have been charged to 81 investigators. A new major initiative in infectious diseases research at our institution has prompted a request that we move the existing FACSCalibur to a BSL-3 facility to provide infectious disease investigators the ability to perform flow cytometry on viable infectious samples. This would seriously interfere with our machine access time for general use and remove our ability to do 4-color analysis required by a growing group of investigators. Thus, we must replace the FACSCalibur before permitting this move. In addition, several investigators are requiring increased number of parameters (5+ colors), more and lower cost Ca++ mobilization experiments, excitation of CFP, and DNA content analysis using Hoechst or DAPI. Thus, in order to meet these increasing demands, replace the FACSCalibur's 4-color capability and general capacity, and provide for future developments we are requesting funds to purchase a four-laser, multi-parameter bench top analytical flow cytometer. At present, we believe the CyAn from DakoCytomation best fulfills our requirements and are requesting, in this application, the majority of funds to purchase the instrument. The University of North Carolina School of Medicine is providing $65,500 towards the purchase in addition to all installation/modification costs ($9,500). The School is also providing considerable on-going support towards the operation of The Flow Cytometry Facility - $80,000/year from The Uneberger Comprehensive Cancer Center and 25% of the Facility Director's salary from The Department of Microbiology and Immunology.