Leica TCS SP2 AOBS Vis-UV Confocal Microscope

描述（由申请人提供）： 该提案要求支持购买Leica TCS SP2 AOBS可见-紫外共聚焦显微镜。这台显微镜将是一种共享资源，对于斯坦福大学医学院几个部门的研究人员正在进行的研究至关重要。主要用户的项目调查了NIH支持的一系列主题，包括：i）细菌（Shapiro）、上皮细胞（纳尔逊）和干细胞（Fuller）中的细胞极性和不对称细胞分裂，ii）细菌（Kaiser）、小鼠胚胎和神经干细胞（Nusse）和配子发生（Tazuke）中的细胞间信号传导和细胞命运，iii）小分子第二信使（Conti）的局部细胞内信号传导机制，iv）神经元和心血管发育的转录调节（Crabtree），v）细胞分裂期间细胞骨架功能和膜运输的机制（Fuller），和vi）减数分裂期间染色体配对和突触的机制（维勒纳夫）。所有这些项目都需要在培养细胞或复杂组织中精确定位蛋白质或其他细胞成分。精确定位蛋白质到特定的细胞隔室和推断蛋白质相互作用的共定位所要求的共享激光扫描共聚焦显微镜提供的能力将是至关重要的，以阐明正常和发病机制，调节和介导的重要发育和细胞事件的调查。上述项目正在进行的高级，中级和最近招聘的教师，谁将合作支持这个共聚焦设施。这种显微镜的分辨率和灵敏度的提高，以及它在单细胞和较厚组织中同时有效成像几种不同荧光的能力，将对这些NIH资助项目的成功至关重要。

DESCRIPTION (provided by applicant): This proposal requests support for the purchase of a Leica TCS SP2 AOBS Vis-UV Confocal Microscope. This microscope, which will be a shared resource, will be crucial for the ongoing research of investigators working in several departments at the Stanford University School of Medicine. The projects of the major users investigate a range of NIH supported topics, including: i) cell polarity and asymmetric cell division in bacteria (Shapiro), epithelial cells (Nelson) and stem cells (Fuller), ii) intercellular signaling and cell fate in bacteria (Kaiser), mouse embryonic and neural stem cells (Nusse), and gametogenesis (Tazuke), iii) mechanisms of localized intracellular signaling by small molecule second messengers (Conti), iv) transcriptional regulation of neuronal and cardiovascular development (Crabtree), v) mechanisms of cytoskeletal function and membrane trafficking during cell division (Fuller), and vi) mechanisms of chromosome pairing and synapsis during meiosis (Villeneuve). All of these projects require precise localization of proteins or other cellular components in cultured cells or complex tissues. The ability to precisely localize proteins to specific cellular compartments and to infer protein interactions by colocalization provided by the requested shared Laser Scanning Confocal microscope will be crucial to elucidating the normal and pathogenetic mechanisms that regulate and mediate the important developmental and cellular events under investigation. The above projects are being undertaken by senior, intermediate, and recently recruited faculty members, who will cooperate in supporting this confocal facility. The enhanced resolution and sensitivity of this microscope, as well as its ability to effectively image several different fluors simultaneously in both single cells and in thicker tissue, will be crucial to the success of these NIH funded projects.