LC/MS/MS Purchase

描述(由申请人提供)：科罗拉多大学医学院将购买一台LC/MS/MS仪器，支持NCRR共享仪器资助计划。为了满足日益增长的多肽和蛋白质的分离和鉴定需求，该仪器是必需的。需求增加的原因是该学院几个系的教员人数增加，蛋白质组学倡议的扩大，以及新的生物分子结构系间项目的建立。所要求的仪器将极大地改善现有资源，并将加速下列系统的研究： 方法糖蛋白质组学的发展；通过选择和结构测定胰酶糖肽来鉴定细胞表面蛋白。(B.本迪亚克) 协调基因调控的结构和机制。(丘吉尔) 蛋白质组学活动中多肽和蛋白质多维液相色谱分离新方法的发展。(R.S.霍奇斯) Pafl/RNA聚合酶II复合体和线粒体RNA聚合酶参与酵母转录的研究。(J.A.Jaehning) 支架相关信号复合体的基因组扫描和鉴定。(G·约翰逊) 监测对生物制药工业重要的重组蛋白在加工和储存过程中的降解/化学修饰。(M.曼宁) 酵母CDC7-Dbf4底物在DNA修复和诱变中的鉴定。(R.Sclafani) 正常和癌细胞内吞作用中与表皮生长因子受体相互作用的蛋白质的鉴定和特性。(A.索尔金) 大鼠脑内缓激肽和P物质多肽类似物/模拟物的设计及P物质受体的克隆(J·M·斯图尔特)

DESCRIPTION (provided by applicant): The University of Colorado School of Medicine will purchase a LC/MS/MS instrument with support for the NCRR Shared Instrumentation Grant Program. This instrument is required to meet the growing demand for the separation and identification of peptides and proteins. The increased demand results from expansion of the faculty in several departments in the School, the expansion of proteomics initiatives, and the establishment of a new interdepartmental Program in Biomolecular Structure. The requested instrument will vastly improve the existing resources and will accelerate research in the following systems: Methods development for glycoproteomics; identification of cell surface proteins through selection and structural determination of their tryptic glycopeptides. (B. Bendiak) Structure and mechanism in coordinated gene regulation. (M. Churchill) Development of new methodology for multidimensional liquid chromatographic separation of peptides and proteins for proteomic initiatives. (R.S. Hodges) Studies of yeast transcription involving Pafl/RNA polymerase II complex and mitochondrial RNA polymerase. (J.A. Jaehning) Genome scanning and identification of scaffold associated signaling complexes. (G. Johnson) Monitoring the degradation/chemical modification of recombinant proteins important to the biopharmaceutical industry during processing and storage. (M. Manning) Identification of yeast Cdc7-Dbf4 substrates in DNA repair and mutagenesis. (R. Sclafani) Identification and characterization of proteins interacting with epidermal growth factor receptor during endocytosis in normal and cancer cells. (A. Sorkin) Design of peptide analogs/mimetic of the hormones bradykinin and substance P and cloning of the substance P receptor in rat brain. (J. M. Stewart)