Confocal Microscope

描述(由申请人提供)：布朗大学NIH研究人员的研究能力受到有限的共焦显微镜访问和技术的影响。在过去的几年里，研究人员不得不推迟实验，前往该地区以外的其他机构进行实验，尽量减少实验方案以适应现有的限制，并将项目的共焦成像外包出去。这一不足极大地造成了NIH对这里研究人员的研究支持的低效利用。对于许多研究人员来说，显微技术的应用也得到了扩展，现有的有限的仪器无法满足这些需求。如果没有得到NCRR的这种支持，我们看不到获得这种工具的其他方法。被确定为主要用户的12名教职员工合作创建了这份资金申请，以支持购买集成的共焦显微镜系统。主要用户组由12个不同的实验室组成，共有86名研究人员。该仪器系统包括以下几部分：1.蔡司LSM 510元共焦显微镜；2.蔡司Axiovert 200 MOT倒置显微镜成像；3.配备Narishige微操作器和埃彭多夫微注射器；4.显微镜工作台温度控制系统。所要求的仪器将大大提高这里所述的广泛的跨学科研究人员的研究效率，并极大地提高该地区的生物成像能力。

DESCRIPTION (provided by applicant): The research capabilities of NIH investigators here at Brown are compromised by limited confocal microscope access and technology. Over the past several years, researchers have had to postpone experiments, travel to other institutions outside of the region to perform experiments, minimize the experimental protocol to accommodate the existing limitations, and farm out the confocal imaging of projects. This deficiency has created greatly inefficient use of NIH research support of investigators here. The applications of microcopy have also expanded for many investigators and the available, limited instrumentation is incapable of meeting these demands. We see no other way of acquiring such instrumentation without receiving this support from the NCRR. Twelve-faculty members, identified as Major Users, have collaborated to create this request for funds to support the acquisition of an integrated confocal microscope system. The Major User Group consists of twelve different laboratories with 86 researchers. The proposed instrument system contains the following: 1. Zeiss LSM 510 META confocal microscope, 2. Imaged through a Zeiss Axiovert 200 MOT inverted microscope, 3. Equipped with Narishige micromanipulators and an Eppendorf microinjector, 4. Microscope stage temperature control system. The requested instrument would significantly enhance the research productivity of the broad, interdisciplinary community of researchers described here, and greatly improve the biological image capabilities in the region.