Fluorescence Activated Cell Sorter

描述（由申请人提供）：本提案要求为Lombardi癌症中心（LCC）流式细胞术/细胞分选共享资源购买FACSVantage SE流式细胞仪的资金。该仪器旨在替换和升级1988年购买的现有和过时的FACStar Plus。该资源维护着校园内研究人员可使用的唯一细胞仪，唯一具有可调谐激光器和可更换发射滤光片的仪器，以及乔治城大学（GU）唯一的荧光激活细胞分选仪。目前的细胞仪FACStar Plus自1992年以来已经停产，而且越来越难以获得维修零件。拟议中的新细胞仪将允许进行中的NIH资助项目所需的更复杂的细胞计数分析，沿着细胞分选能力的巨大改进。TurboSort功能将允许研究人员分选出大量细胞或分离出足够数量的罕见事件群体，由于时间限制，这些群体现在无法进行分选。QuadraSort功能在分选多个群体时将是有价值的，特别是从细胞数量有限的样品中。一个额外的功能将允许非矩形人口的排序。在23个主要用户中，目前有8个项目需要当前仪器无法提供的功能。其他项目将因这些特点而得到加强。40多名PI将大大受益于可靠的最先进的血细胞计数器。11个部门的调查员大量使用该资源。2001年，43名调查人员利用该设施进行了750多项实验。目前，有超过29名研究人员与同行评审的NIH资助的项目利用该设施沿着与5名研究人员与同行评审的资助项目从其他资金来源。该资源成立于1988年，由癌症中心资金，NCI CCSG赠款支持和用户费用的组合支持。 该资源由Karen Creswell博士领导，他在流式细胞术方面有12年的经验，并将担任主要仪器操作员。一个咨询委员会负责监督准入、费用、政策，并为未来的增长提出建议。LCC致力于支持这一资源，以确保持续的维护，优质的服务和广泛的访问GU社区。

DESCRIPTION (provided by applicant): This proposal requests the funds to purchase a FACSVantage SE flow cytometer for the Lombardi Cancer Center (LCC) Flow Cytometry/Cell Sorting Shared Resource. This instrument is intended as a replacement and upgrade for an existing and outdated FACStar Plus purchased in 1988. The Resource maintains the only cytometers accessible to researchers on the campus, the only instrument with tunable lasers and exchangeable emission filters as well as the only fluorescence activated cell sorters at Georgetown University (GU). The current cytometer, the FACStar Plus, has been out of production since 1992 and it is becoming more difficult to obtain repair parts. The proposed new cytometer will allow more complex multicolor analyses required for ongoing NIH funded projects along with great improvements in cell sorting capabilities. The TurboSort feature will allow investigators to sort out large numbers of cells or to isolate sufficient quantities of rare event populations, which are now not feasible to sort due to time constraints. The QuadraSort capability will be valuable in sorting multiple populations, particularly from samples with limited cell numbers. An additional feature will allow for the sorting of nonrectangular populations. Of the 23 major users, 8 currently have projects which require capabilities not available on the present instrumentation. Other projects would be enhanced by these features. More than 40 PIs would greatly benefit from the availability of a reliable state-of-the-art cytometer. The Resource is heavily utilized by investigators from 11 departments. In 2001, 43 investigators utilized the facility for more than 750 experiments. Currently, there are more than 29 investigators with peer-reviewed NIH funded projects utilizing the facility along with 5 investigators with peer reviewed funded projects from other funding sources. The Resource, established in 1988, is supported by a combination of Cancer Center funding, NCI CCSG grant support, and user fees. The Resource is headed by Dr. Karen Creswell, who has 12 years of experience in flow cytometry, and who will serve as the primary instrument operator. An advisory committee oversees access, fees, policies, and makes recommendations for future growth. The LCC is committed to support this Resource to ensure continued upkeep, excellent service and wide access to the GU community.