Core--Microarray Facility

微阵列共享资源为癌症中心的成员以及其他大学研究人员提供微阵列服务。这些服务包括打印微阵列幻灯片、在安捷伦生物分析仪上分析样本以确定RNA质量、用Cy3和Cy5荧光染料标记样本、微阵列杂交、微阵列扫描和数据捕获、数据分析以及cDNA克隆分发。 该设施已经购买了几件关键的仪器，包括一台amersham Lucei阵列Spotter、一台Axon 4000B扫描仪、一台安捷伦生物分析仪、两台BioMicro Systems毛伊杂交装置、一台Packard Instruments多探针II液体处理机和一台MJ Research Tetrad热循环仪。使用BioDiscovery Imagene软件对扫描的阵列进行量化；微阵列资源的客户可以使用GeneSight软件对其阵列进行基因挖掘和数据分析。微阵列实验室信息管理系统用于跟踪克隆、载玻片、工作流程、样本、实验请求和数据。微阵列资源已经获得了大量的cDNA克隆，包括人类(42,000个)、小鼠(34,000个)、酵母ORF(6,000个)、酵母基因间序列(6,000个)、果蝇(12,000个)、指环虫 (4000)和Newt(1500)。每年大约进行2000次微阵列分析。微阵列资源提议加强以下领域的服务：扩大目前的克隆收集，增强微阵列数据存储能力，以及提高对微阵列数据分析和解释的支持水平。为了扩大我们提供从用于癌症研究的实验生物体中提取的微阵列的能力，我们希望获得斑马鱼寡头文库的收藏。为了提高微阵列资源的数据存储能力，我们计划购买一台Iomega NAS P850m数据存储服务器(在Informatics共享资源中申请资金)。该设施的另一个重点领域将是在实验设计、分析和数据解释方面提供咨询。为了实现这一目标，我们增加了一名工作人员，专注于在这些领域向我们的客户提供咨询。

The Microarray Shared Resource provides microarray services to members of the Cancer Center as well as to other University investigators. These services include printing of microarray slides, analysis of samples on an Agilent BioAnalyzer to define RNA quality, labeling of samples with Cy3 and Cy5 fluorescent dyes, microarray hybridization, microarray scanning and data capture, data analysis, and cDNA clone distribution. The facility has acquired several key pieces of instrumentation including an Amersham Lucidea Array Spotter, an Axon 4000B Scanner, an Agilent BioAnalyzer, two BioMicro Systems MAUI hybridization units, a Packard Instruments Multiprobe II liquid handler, and an MJ Research Tetrad thermal cycler. Scanned arrays are quantified using BioDiscovery ImaGene software; GeneSight software is available to clients of the Microarray Resource for gene mining and data analysis of their arrays. A microarray laboratory information management system is used to track clones, slides, workflow, samples, experiment requests and data. The Microarray Resource has acquired an extensive collection of cDNA clones that includes human (42,000), mouse (34,000), yeast ORFs (6,000), yeast intergenic sequences (6,000), Drosophila (12,000), Planaria (4,000) and newt (1,500). On an annual basis, approximately 2000 microarray analyses are performed. The Microarray Resource proposes to enhance services in the following areas: expansion of the current clone collections, enhancement of microarray data storage capability, and increased levels of support for microarray data analysis and interpretation. To expand our ability to offer microarrays derived from experimental organisms actively used in cancer studies, we hope to acquire a zebrafish oligo library collection. To improve data storage capabilities of the Microarray Resource, we plan to acquire an Iomega NAS P850m data storage server (funding requested in the Informatics Shared Resource). An additional area of emphasis at the facility will be to provide consultation in experimental design, analysis and interpretation of data. To accomplish this goal, we have added a staff member to focus on consulting with our clients in these areas.