System for selection and identification of antibodies

描述（由申请人提供）：这是一份建立高通量和可平行化方法的技术原理证明的提案，用于产生完全人单克隆抗体。我们描述了一种新版本的酵母双杂交（Y2H）系统的开发，用于识别与诱饵蛋白相互作用的抗体，一种新的迭代方法，用于增加序列空间的搜索，以及一种快速，廉价和准确地识别引起抗体-抗原相互作用的特定抗体序列的方法。我们获得的抗体被设计成克隆到框架中，用于立即开发临床有用的抗体药物。

DESCRIPTION (provided by applicant): This is a proposal to establish the technological proof-of-principle for a high-throughput and parallizable means for generating fully human monoclonal antibodies. We describe the development of a new version of the yeast two-hybrid (Y2H) system to identify antibodies that interact with a bait protein, a novel iterative method for increased searching of sequence space, and a method with which to quickly, cheaply and accurately identify specific antibody sequences giving rise to antibody-antigen interactions. The antibodies we derive are designed to be cloned into frameworks for the immediate development of clinically useful antibody drugs.