Radio-labelled responsive fluorophores to elucidate the role of MMP in ageing

基质金属蛋白酶（MMPs）在包括衰老在内的多种生物过程中发挥着重要作用。在这个项目中，将开发一个多功能探针的新平台，用于详细研究MMP在年龄相关疾病中的作用。探针将由有机荧光团共价连接到pet示踪剂，并通过MMP可切割肽序列锚定在黑洞猝灭剂（BHQ）上。pet示踪剂将允许对探针在细胞基质中的扩散进行空间监测，而BHQ将在被MMP切割时增加时间分辨率，从而“开启”荧光。

Matrix metalloproteinases (MMPs) play an essential role in a variety of biological processes,including ageing. Within this project, a new platform of multifunctional probes for the detailedstudy of MMP's role in age-related diseases will be developed. The probes will consist of anorganic fluorophore covalently linked to a PET-tracer and anchored via an MMP cleavablepeptide sequence to a black-hole quencher (BHQ). The PET-tracer will allow for the spatialmonitoring of the probe's diffusion in the cellular matrix, while the BHQ will add temporalresolution when cleaved by MMP, thereby "turning-on" the fluorescence.