Analysis of the Yeast Cell Cycle Transcriptional Program

描述(申请人提供)：细胞周期控制被组织成一个循环，因此周期可能会反复发生。这些调控有几种：转录调控、蛋白降解调控、蛋白激酶调控等。微阵列技术的发展使得研究所有类型的转录调控变得更加容易和有效。事实上，关于细胞周期转录调控的微阵列研究提供了丰富的数据。在这项提案中，微阵列技术将被用于继续研究酵母细胞周期的转录控制。特别是，将寻求对细胞周期控制的循环性质的概述。在目标1中，我们将完成将细胞周期调控基因分配给它们的调控转录因子的任务。在目标2中，我们将发现细胞周期基因表达的时间是如何决定的，并将定义在该周期中组合控制的程度和机制。在目标3中，我们将验证组蛋白乙酰化状态的振荡是细胞周期转录控制的主要机制的假设。

DESCRIPTION (provided by applicant): Cell cycle controls are organized into a loop, so that cycles may occur over and over again. These controls are of several kinds: transcriptional controls, proteolytic controls, protein kinase controls, etc. The development of microarray technology has now made it far easier and more efficient to investigate all kinds of transcriptional controls. Indeed, microarray studies on the transcriptional regulation of the cell cycle have provided a wealth of data. In this proposal, microarray technology will be used to continue the study of the transcriptional control of the yeast cell cycle. In particular, an overview of the circular nature of cell cycle controls will be sought. In Aim 1, we will complete the task of assigning cell cycle regulated genes to their regulating transcription factors. In Aim 2, we will find out how the time at which cell cycle genes are expressed is determined, and we will define the extent and mechanisms of combinatorial control in the cycle. In Aim 3, we will test the hypothesis that an oscillation in the state of histone acetylation is a major mechanism of cell cycle transcriptional control.