Real time sequencing for assembly of complex genomic regions using selective sequencing

我的研究兴趣集中在计算和遗传技术在生命科学问题上的应用。我发现种群遗传学和进化发育生物学特别令人着迷，因为它们结合了理解基因如何相互作用来创造生命，以及遗传差异如何导致多样性。为了进一步探索这些领域，我承担了一个由Alistair McGregor教授指导的学位论文项目，在那里我研究了蜘蛛Parasteatoda tepidariorum中平行基因的时空表达；这个项目主要使用分子技术来克隆和生成杂交实验中使用的感兴趣基因的探针。此外，我还在与Daniela Santos-Nunes博士进行一项独立研究，使用从混合测序获得的种群基因组数据来识别模拟果蝇和毛果果蝇的多态位点，并开发一个网络应用程序，允许对这两个物种进行核苷酸逐个比较。我是一个有组织、有进取心的学生。作为我本科课程的一部分，我从事的研究为我提供了分子生物学和生物信息学方面的一系列技能；并通过参加每周的研究研讨会和与来自英国各地的研究人员的讨论，使我获得了一系列技能。我对学术界的研究进展有了深入的了解。此外，我在IT服务部门的职位培养了我的一般技术知识和解决问题的技能，同时也让我获得了编写技术文档的经验。

My research interests are focused upon the application of computational and genetic techniques to problems in the life sciences. I have found population genetics and evolutionary-developmental biology to be particularly fascinating as they combine understanding how genes interact to create life and, how genetic differences lead to diversity. To further explore these areas I undertook a dissertation project, supervised by Professor Alistair McGregor, where I investigated the spatiotemporal expression of paralogous genes in the spider Parasteatoda tepidariorum; this project primarily used molecular techniques to clone and generate probes for genes of interest that were used in hybridisation experiments. Furthermore, I am also pursuing an independent study with Dr Daniela Santos-Nunes to identify polymorphic sites in Drosophila simulans and D. mauritiana using population genomic data obtained from pooled sequencing and to develop a web application that allows nucleotide by nucleotide comparison for both species. I am an organised and motivated student. The research that I have undertaken, as part of my undergraduate course, has provided me with a range of skills in both molecular biology and bioinformatics; and, through attending weekly research seminars and discussions with researchers from across the UK. I have gained insight into how research progresses in academia. Moreover, my position with the IT Services department has developed my general technical knowledge and problem solving skills, while allowing me to gain experience writing technical documentation.