Microarray Program for NHLBI Investigators

描述(由申请人提供)： 多基因组计划的进展，包括致力于人类基因组的工作成果，为在全球基因表达研究中使用精确定义和日益完善的注释的序列提供了无与伦比的机会。允许通过基因表达微阵列进行高通量转录组评估的技术是成熟和强大的，并为临床和实验室科学家提供了一种新的发现科学模式。生物信息学的工具越来越强大，一些工具可以应用于不同的微阵列平台。俄勒冈健康与科学大学(OHSU)正在积极使用微阵列技术。本申请的首席研究员和联合首席研究员都是OHSU的研究所主任和NHLBI资助的科学家，他们积极利用基因微阵列技术，领导了OHSU基因微阵列共享资源(GMSR)的规划和开发。这一规划活动始于两年前，迄今已承诺投入220万美元建设一座设施。共享资源由三个核心实验室组成：Affymetrix微阵列核心(AMC)、斑点微阵列核心(SMC)和生物信息学/生物统计学核心(BBC)。我们在此建议为NHLBI研究人员建立俄勒冈健康与科学大学(OHSU)微阵列计划。该计划将支持OHSU GMSR的扩展和增强，将支持NHLBI研究人员开展有功项目，将为所有NHLBI资助的研究人员提供微阵列研究设计指导和数据分析支持，并将实施针对科学家、学生、博士后研究员和研究助理的培训和教育计划。最初，微阵列计划将支持由NHLBI资助的五个项目，这些项目涉及造血、淋巴肿大、脑血管疾病、冠状动脉疾病和干细胞生物学等领域。然而，我们的长期目标是尽可能多地增加由NHLBI资助的科学家对这项技术的使用，并招募新的研究人员到心血管、肺部和血液疾病领域。因此，试点项目将由大学通过其研究所和中心提供支持。此外，在整个授权期内，OHSU NHLBI微阵列计划将为OHSU所有NHLBI资助的研究人员提供教育推广服务，并将保持一个持续的论坛，用于技术更新、项目讨论和数据共享。

DESCRIPTION (provided by applicant): The progress of multiple genome projects, including the results of work devoted to the human genome, has provided unparalleled opportunities for the use of precisely defined and increasingly well-annotated sequences in studies on global gene expression. Technologies that permit high throughput transcriptome assessments by gene expression microarrays are well established and robust and have provided a new model of discovery science for clinical and bench scientists alike. Tools for bioinformatics are increasingly strong, and some can be applied across different microarray platforms. Microarray technologies are in active use at Oregon Health & Science University (OHSU). The Principal Investigator and co-Principal Investigator of this application, who are both Institute Directors at OHSU and NHLBI-funded scientists actively utilizing gene microarray technology, led the planning and development of the OHSU Gene Microarray Shared Resource (GMSR). This planning activity began two years ago, and has resulted in a facility to which $2.2m has been committed to date. The shared resource consists of three core laboratories: the Affymetrix Microarray Core (AMC), the Spotted Microarray Core (SMC), and the Bioinformatics/ Biostatistics Core (BBC). We propose herein to establish the Oregon Health and Science University (OHSU) Microarray Program for NHLBI Investigators. The program will support expansion and enhancement of the OHSU GMSR, will support the conduct of meritorious projects of NHLBI investigators, will provide microarray study design guidance and data analysis support for all NHLBI-funded investigators, and will implement a training and education program for scientists, students, post-doctoral fellows, and research associates. Initially, the Microarray Program will support five NHLBI-funded projects in the fields of hematopoiesis, lymphomagenesis, cerebro-vascular disease, coronary artery disease, and stem cell biology. However, the long-term objectives are to increase the use of this technology by as many of our NHLBI-funded scientists as possible and to recruit new investigators to the fields of cardiovascular, pulmonary, and blood diseases. Accordingly, pilot projects will be supported by the University through its institutes and centers. Moreover, throughout the grant period the OHSU NHLBI Microarray Program will provide educational outreach services available to all NHLBI funded investigators at OHSU and will maintain an ongoing forum for technological updates, project discussion, and data sharing.